Phagocytosis of fibronectin and collagens type I, III, and V by human gingival and periodontal ligament fibroblasts in vitro.
Phagocytosis of fibronectin and collagens type I, III, and V by human gingival and periodontal ligament fibroblasts in vitro.
复制标题
人牙龈和牙周膜成纤维细胞对纤连蛋白和 I、III 和 V 型胶原的体外吞噬作用。
DOI：
10.1902/jop.2001.72.10.1340
复制
发表时间：
2001
影响因子：
4.3
通讯作者：
W. Beertsen
中科院分区：
文献类型：
--
作者：
M. T. V. D. Pauw;T. V. D. Bos;Vincent Everts;W. Beertsen
背景
电子显微镜研究表明，牙周膜（PDL）中成纤维细胞中含胶原的空泡的体积密度高于牙龈。这种差异是否反映了这些组织中细胞吞噬能力的内在差异尚不清楚。
方法
PDL和牙龈成纤维细胞从受试者中分离出来，并在相同的条件下，在荧光珠涂有胶原蛋白I型，III型或V型或纤连蛋白的存在下培养。对照珠用牛血清白蛋白或不构成PDL和牙龈的细胞外基质的一部分的釉质基质蛋白混合物包被。在不同的时间间隔（1至24小时）后，通过流式细胞术评估具有内化珠粒的细胞的百分比。由于碱性磷酸酶活性已被认为在胶原蛋白吞噬作用中起作用，因此测定了所有细胞群中该酶的活性。
结果
结果表明，细胞内化蛋白质包被的珠粒的百分比的顺序如下：纤连蛋白> I型胶原> III > V。I型胶原包被的珠粒的内化分别超过用牛血清白蛋白或釉基质蛋白包被的珠粒的内化6倍和3倍。牙周膜和牙龈成纤维细胞之间的胶原吞噬活性没有差异，胶原吞噬和碱性磷酸酶活性之间没有关系。
结论
我们的结论是，在PDL和牙龈之间的胶原蛋白的吞噬作用的差异，在体内观察到的，是不太可能解释这些细胞的内在吞噬能力。
BACKGROUND
Electron microscopic studies have suggested that the volume density of collagen-containing vacuoles in fibroblasts is higher in the periodontal ligament (PDL) than in the gingiva. Whether this difference reflects intrinsic differences in phagocytic capacity among the cells in these tissues is not known.
METHODS
PDL and gingival fibroblasts were isolated from subjects and cultured under identical conditions in the presence of fluorescent beads coated with collagen type I, III, or V or fibronectin. Control beads were coated with bovine serum albumin or an enamel matrix protein mixture that does not constitute part of the extracellular matrix of PDL and gingiva. After various time intervals (1 to 24 hours), the percentage of cells that had internalized beads was assessed by flow cytometry. Since alkaline phosphatase activity has been suggested to play a role in collagen phagocytosis, the activity of this enzyme was determined for all cell populations.
RESULTS
The results demonstrated the following order in the percentage of cells internalizing protein-coated beads: fibronectin > collagen type I > III > V. Internalization of collagen type I-coated beads exceeded that of beads coated with bovine serum albumin or enamel matrix proteins by 6 and 3 times, respectively. No differences were observed in collagen phagocytic activity between PDL and gingival fibroblasts, and no relationship could be demonstrated between collagen phagocytosis and alkaline phosphatase activity.
CONCLUSIONS
We conclude that differences in collagen phagocytosis between PDL and gingiva, as observed in vivo, are not likely to be explained in terms of intrinsic phagocytic capacities of these cells.
登录
查看更多内容
比格犬牙龈结缔组织中纤连蛋白的定位。
影响因子：
4.3
作者：
Cho,MI;Lee,YL;Garant,PR
通讯作者：
Garant,PR
DOI：
10.1006/bbrc.1995.1003
发表时间：
1995-01-05
影响因子：
3.1
作者：
MURRELL, GAC;JANG, D;WILLIAMS, RJ
通讯作者：
WILLIAMS, RJ
DOI：
10.1006/bbrc.1996.0125
发表时间：
1996-01-26
影响因子：
3.1
作者：
Trachtman, H;Futterweit, S;Singhai, PC
通讯作者：
Singhai, PC
影响因子：
3.5
作者：
Pamela S. Howard;Umberto Kucich;Rita Taliwal;Jonathan Korostoff
通讯作者：
Pamela S. Howard;Umberto Kucich;Rita Taliwal;Jonathan Korostoff
影响因子：
20.1
作者：
Ziche, M;Parenti, A;Presta, M
通讯作者：
Presta, M