Effects of agarose mould compliance and surface roughness on self-assembled meniscus-shaped constructs
Effects of agarose mould compliance and surface roughness on self-assembled meniscus-shaped constructs
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DOI：
10.1002/term.191
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发表时间：
2009-10-01
影响因子：
3.3
通讯作者：
Athanasiou, Kyriacos A.
中科院分区：
文献类型：
--
作者：
Gunja, Najmuddin J.;Huey, Dan J.;Athanasiou, Kyriacos A.
半月板是一种纤维软骨组织，对膝关节内的负荷模式至关重要。如果半月板结构受损，由于组织内部的血管分布有限，愈合的机会很小。已经采用了几种组织工程技术来模拟半月板的复杂几何形状。其中，采用琼脂糖模具的自组装无支架方法避免了与支架使用相关的缺点，同时仍然允许形成坚固的组织。在该实验中，检查了两个因素，琼脂糖百分比和模具表面粗糙度，以努力一致地获得具有足够几何特性的构建体。将AC和MC（50：50比例）的共培养物在由1%或2%琼脂糖组成的光滑或粗糙模具中培养4周。形态学结果表明，在1%琼脂糖模具中形成的构建体，特别是光滑模具，能够在4周的培养期内保持其形状。在光滑威尔斯孔中观察到胶原II：胶原I比率、总胶原、GAG以及拉伸和压缩特性显著增加。粗糙威尔斯孔中每个构建体的细胞数较高。总体而言，观察到琼脂糖表面的拓扑结构可能能够影响该表面上的细胞的表型性质，其中光滑表面支持更多的软骨细胞表型。此外，由1%琼脂糖制成的威尔斯孔由于其较高的顺应性而能够潜在地防止结构屈曲。版权所有（C）2009约翰威利父子有限公司
The meniscus is a fibrocartilaginous tissue that is critically important to the loading patterns within the knee joint. if the meniscus structure is compromised, there is little chance of healing, due to limited vascularity in the inner portions of the tissue. Several tissue-engineering techniques to mimic the complex geometry of the meniscus have been employed. Of these, a self-assembly, scaffoldless approach employing agarose moulds avoids drawbacks associated with scaffold use, while still allowing the formation of robust tissue. In this experiment two factors were examined, agarose percentage and mould surface roughness, in an effort to consistently obtain constructs with adequate geometric properties. Co-cultures of ACs and MCs (50:50 ratio) were cultured in smooth or rough moulds composed of 1% or 2% agarose for 4 weeks. Morphological results showed that constructs formed in 1% agarose moulds, particularly smooth moulds, were able to maintain their shape over the 4 week culture period. Significant increases were observed for the collagen II: collagen I ratio, total collagen, GAG and tensile and compressive properties in smooth wells. Cell number per construct was higher in the rough wells. Overall, it was observed that the topology of an agarose surface may be able to affect the phenotypic properties of cells that are on that surface, with smooth surfaces supporting a more chondrocytic phenotype. In addition, wells made from 1% agarose were able to prevent construct buckling potentially, due to their higher compliance. Copyright (C) 2009 John Wiley & Sons, Ltd.