Molecular Capture in Protein Nanotubes
Molecular Capture in Protein Nanotubes
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DOI：
10.1021/nn901474y
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发表时间：
2010-01-01
期刊：
影响因子：
17.1
通讯作者：
Komatsu, Teruyuki
中科院分区：
文献类型：
--
作者：
Qu, Xue;Komatsu, Teruyuki
我们描述了具有可控配体结合亲和力和尺寸选择性的蛋白质纳米管的分子捕获特性。这些实用的生物支架是通过交替地将蛋白质和相反电荷的聚(氨基酸)组装到纳米多孔聚碳酸酯(PC)膜(孔径为400 nm)中，然后溶解模板来制备的。管壁通常由六层聚L精氨酸和人血清白蛋白组成[(聚乳酸/人血清白蛋白)(3)]。使用高相对分子质量的聚乳酸(M-w=约70000)可生产出坚固的纳米管，这些纳米管可作为冻干粉末使用。(聚乳酸/人血清白蛋白)(3)纳米管在水中膨胀很大，尽管外径几乎没有改变。铀酰离子、3，3‘-二乙基硫代碳菁碘和锌(II)原卟啉IX(ZnPP)被结合到圆柱壁的HSA组分上。类似的纳米管；包含重组人血清白蛋白突变体[RHSA(His)]，它与锌PP具有很强的结合亲和力，更紧密地捕获了这一配体。此外，过量的肉豆蔻酸的加入通过配体替换反应从管子中释放出了锌PP。以单层亲和素为内表面的杂化纳米管有效地捕获了FITC-生物素。当生物素标记的纳米颗粒尺寸足够小，可以进入毛孔时，它们也会被结合到管子中。随后的透射电子显微镜观察显示，在一维空间内部有一条负载纳米颗粒(100 Nm)的线条。
We describe molecular capturing properties of protein nanotubes with a controllable ligand binding affinity and size selectivity. These practical biocylinders were prepared using an alternating layer-by-layer (LbL) assembly of protein and oppositely charged poly(amino acid) into the nanoporous polycarbonate (PC) membrane (pore diameter, 400 nm), with subsequent dissolution of the template. The tube wall typically comprises six layers Of poly-L-arginine (PLA) and human serum albumin (HSA) [(PLA/HSA)(3)]. Use of high molecular weight PLA (M-w = ca. 70 000) yielded robust nanotubes, which are available as lyophilized powder. The (PLA/HSA)(3) nanotubes swelled considerably-in water, although the outer diameter was almost unaltered. Uranyl ion, 3,3'-diethylthiacarbocyanine iodide, and-zinc(II) protoporphyrin IX (ZnPP) were bound to the HSA component in the cylinder wall. Similar nanotubes; comprising recombinant HSA mutant [rHSA(His)], which has a strong binding affinity for ZnPP, captured this ligand more tightly. Furthermore, addition of excess myristic acid released ZnPP from the tubes through a ligand replacement reaction. The hybrid nanotubes bearing a single avidin layer as an internal surface captured FITC-biotin efficiently. Biotin-labeled nanoparticles are also incorporated into the tubes when their particle size is sufficiently small to enter the pores. Subsequent TEM observation revealed a line of loaded nanoparticles (100 nm) in the one-dimensional space interior.