Meiotic spindle pole bodies acquire the ability to assemble the spore plasma membrane by sequential recruitment of sporulation-specific components in fission yeast
Meiotic spindle pole bodies acquire the ability to assemble the spore plasma membrane by sequential recruitment of sporulation-specific components in fission yeast
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DOI：
10.1091/mbc.e08-02-0118
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发表时间：
2008-06-01
影响因子：
3.3
通讯作者：
Nakamura, Taro
中科院分区：
文献类型：
--
作者：
Nakase, Yukiko;Nakamura-Kubo, Michiko;Nakamura, Taro
粟酒裂殖酵母的纺锤体极体（SPB）是减数分裂过程中前孢子膜（FSM）组装所必需的。在 FSM 从头生物发生之前，减数分裂 SPB 形成外部斑块，这一事件称为 SPB 修饰。 SPB 的组成成分 Spo15 在 SPB 修饰和孢子形成中起着不可或缺的作用。在这里，我们分析了两个孢子形成特异性基因 spo13(+) 和 spo2(+)，它们不是减数分裂核分裂进展所必需的，但对于孢子形成至关重要。 Spo13 是一种 16 kDa 卷曲螺旋蛋白，Spo2 是一种 15 kDa 非保守蛋白。 Spo13 和 Spo2 都与减数分裂 SPB 特异性相关。相应的缺失突变体是可行的，但在 SPB 修饰和 FSM 形成的开始方面存在缺陷。 Spo13 和 Spo2 位于 SPB 的细胞质侧，与新生的 FSM 紧密接触。 Spo13 向 SPB 的定位依赖于 Spo15 和 Spo2； Spo2 仅依赖于 Spo15，这表明它们对 SPB 的招募受到严格控制。 Spo2 在物理上与 Spo15 和 Spo13 相关，但 Spo13 和 Spo15 不直接相互作用。总而言之，这些观察结果表明 Spo2 在减数分裂过程中被招募到 SPB，然后协助 Spo13 定位到 SPB 的外表面。
The spindle pole body (SPB) of Schizosaccharomyces pombe is required for assembly of the forespore membrane (FSM) during meiosis. Before de novo biogenesis of the FSM, the meiotic SPB forms outer plaques, an event referred to as SPB modification. A constitutive SPB component, Spo15, plays an indispensable role in SPB modification and sporulation. Here, we analyzed two sporulation-specific genes, spo13(+) and spo2(+), which are not required for progression of meiotic nuclear divisions, but are essential for sporulation. Spo13 is a 16-kDa coiled-coil protein, and Spo2 is a 15-kDa nonconserved protein. Both Spo13 and Spo2 specifically associated with the meiotic SPB. The respective deletion mutants are viable, but defective in SPB modification and in the onset of FSM formation. Spo13 and Spo2 localized on the cytoplasmic side of the SPB in close contact with the nascent FSM. Localization of Spo13 to the SPB was dependent on Spo15 and Spo2; that of Spo2 depended only on Spo15, suggesting that their recruitment to the SPB is strictly controlled. Spo2 physically associated with both Spo15 and Spo13, but Spo13 and Spo15 did not interact directly. Taken together, these observations indicate that Spo2 is recruited to the SPB during meiosis and then assists in the localization of Spo13 to the outer surface of the SPB.