Changes in periodontal health status are associated with bacterial community shifts as assessed by quantitative 16S cloning and sequencing
Changes in periodontal health status are associated with bacterial community shifts as assessed by quantitative 16S cloning and sequencing
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DOI：
10.1128/jcm.00317-06
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发表时间：
2006-10-01
影响因子：
9.4
通讯作者：
Griffen, Ann L.
中科院分区：
文献类型：
--
作者：
Kumar, Purnima S.;Leys, Eugene J.;Griffen, Ann L.
牙龈沟包含一个复杂的生态系统，其中包括许多未培养的细菌。了解这个生态系统在健康和疾病之间的转变的动态对于促进我们对牙周炎细菌病因学的理解非常重要。这项纵向研究的目的是检查细菌定植在龈沟的稳定性，并探讨微生物组成的变化和牙周健康状况的变化之间的关系，使用一个全面的，定量的，文化的独立的方法。收集了24名受试者2年以上的龈下菌斑样本和牙周数据。使用定量核糖体16 S克隆和测序分析基线和2年斑块样本。10名受试者在2年内保持牙周健康，7名受试者的牙周健康恶化，7名受试者表现出临床改善。细菌稳定性在健康、临床稳定的受试者中最高，在牙周状况恶化的受试者中最低（P = 0.01）。在临床状态发生变化的受试者中，也观察到更多的物种丢失或获得（P = 0.009）。这提供了证据表明，牙周状况的变化伴随着细菌群落的变化。基于这些数据，微生物稳定性的测量可能有助于临床诊断和预后。关于个别物种，增加水平的未培养的革兰氏阴性菌和龈下细菌群落的最常见成员韦荣氏菌属口腔克隆X042，与牙周健康（P = 0.04），这表明这是一个重要的有益物种。Filifactoralocis是一种革兰氏阳性厌氧菌，在患病受试者中的水平较高（P = 0.01）。
The gingival sulcus contains a complex ecosystem that includes many uncultivated bacteria. Understanding the dynamics of this ecosystem in transitions between health and disease is important in advancing our understanding of the bacterial etiology of periodontitis. The objective of this longitudinal study was to examine the stability of bacterial colonization in the gingival crevice and to explore the relationship between shifts in microbial composition and changes in periodontal health status using a comprehensive, quantitative, culture-independent approach. Subgingival plaque samples and periodontal data were collected from 24 subjects over 2 years. Baseline and 2-year plaque samples were analyzed using quantitative ribosomal 16S cloning and sequencing. Ten subjects remained periodontally healthy over 2 years, the periodontal health of seven subjects worsened, and seven subjects showed clinical improvement. Bacterial stability was greatest among healthy, clinically stable subjects and lowest for subjects whose periodontal status worsened (P = 0.01). Higher numbers of species lost or gained were also observed for subjects whose clinical status changed (P = 0.009). This provides evidence that a change in periodontal status is accompanied by shifts within the bacterial community. Based on these data, measures of microbial stability may be useful in clinical diagnosis and prognosis. Regarding individual species, increases in levels of the uncultivated phylotype Veillonella sp. oral clone X042, a gram-negative bacterium and the most common member of the subgingival bacterial community, were associated with periodontal health (P = 0.04), suggesting that this is an important beneficial species. Filifactor alocis, a gram-positive anaerobe, was found at higher levels in subjects with disease (P = 0.01).