'Candidatus Protochlamydia amoebophila', an endosymbiont of Acanthamoeba spp.
'Candidatus Protochlamydia amoebophila', an endosymbiont of Acanthamoeba spp.
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DOI：
10.1099/ijs.0.63572-0
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发表时间：
2005-09-01
影响因子：
2.8
通讯作者：
Horn, M
中科院分区：
文献类型：
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作者：
Collingro, A;Toenshoff, ER;Horn, M
专性胞内球形细菌UWE 25是阿米巴属的共生体，先前基于衣原体样发育周期及其16 S rRNA基因序列的存在，将其鉴定为与衣原体相关。其完整基因组序列的分析表明，UWE 25显示出衣原体的许多特征，包括对宿主衍生代谢物的依赖性，细胞包膜的组成以及作为能量寄生虫在其真核宿主细胞内茁壮成长的能力。44个核糖体蛋白的系统发育分析进一步证实了UWE 25与衣原体的亲缘关系。根据16 S rRNA、23 S rRNA和核糖核酸内切酶P RNA基因的比较分析，UWE 25属于衣原体科（帕拉chlamydiaceae）。不同的T与其最近的亲戚棘阿米巴副衣原体Bn-9（T）（7（.）1，9（.）7和28（.）8%，分别），观察到在这项分析证明其分类在一个新的属。因此，拟将阿米巴属共生体UWE 25（=ATCC PRA-7）命名为“嗜阿米巴原衣原体”。
The obligately intracellular coccoid bacterium UWE25, a symbiont of Acanthamoeba spp., was previously identified as being related to chlamydiae based upon the presence of a chlamydia-like developmental cycle and its 16S rRNA gene sequence. Analysis of its complete genome sequence demonstrated that UWE25 shows many characteristic features of chlamydiae, including dependency on host-derived metabolites, composition of the cell envelope and the ability to thrive as an energy parasite within the cells of its eukaryotic host. Phylogenetic analysis of 44 ribosomal proteins further confirmed the affiliation of UWE25 to the 'Chlamydiae'. Within this phylum, UWE25 could be assigned to the family Para chlamydiaceae based on comparative analyses of the 16S rRNA, 23S rRNA and endoribonuclease P RNA genes. The distinct T dissimilarities from its closest relative, Parachlamydia acanthamoebae Bn-9(T) (7(.)1,9(.)7 and 28(.)8%, respectively), observed in this analysis justify its classification in a new genus. Therefore, the name 'Candidatus Protochlamydia amoebophila' is proposed for the designation of the Acanthamoeba sp. symbiont UWE25 (=ATCC PRA-7).