Blends of poly-(ε-caprolactone) and polysaccharides in tissue engineering applications
Blends of poly-(ε-caprolactone) and polysaccharides in tissue engineering applications
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DOI：
10.1021/bm0500805
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发表时间：
2005-07-01
影响因子：
6.2
通讯作者：
Giusti, P
中科院分区：
文献类型：
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作者：
Ciardelli, G;Chiono, V;Giusti, P
生物人工共混物的聚-（ε-己内酯）（PCL）与多糖（淀粉，S;右旋糖酐，D;或结冷胶，G）采用溶液-沉淀法制备了PCL/S、PCL/D、PCL/G（90.9/9.1 wt（FTIR-ATR）、光学显微镜（OM）、广角X射线衍射分析（WAXD）。和热重分析（TGA）。差示扫描量热分析表明，多糖降低了聚己内酯的结晶度，并具有成核作用，OM分析也证实了这一点。对PCL和共混物样品进行Hoffman-Weeks分析，以计算其平衡熔融温度（T-m（0））。WAXD表明PCL和共混物的结晶晶胞类型相同。rTIR-ATR未证明混合组分之间的相互作用。热稳定性受多糖种类的影响。通过低温研磨由共混物制备微粒（< 125 μ m），并通过扫描电子显微镜分析（SEM）表征。选择性激光烧结（SLS），一种新的快速成型技术的支架制造，应用烧结共混微粒根据PC设计的二维几何形状（条和2 × 2 mm（2），正方形网格）。烧结的最佳实验条件的建立和激光束参数（光束速度，BS和功率，P）被发现为每个共混物组合物。通过SEM分析烧结体的形貌，发现其取决于烧结粉末的形貌。通过化学成像（CI）、FTIR-ATR、DSC和接触角分析来分析烧结样品。FTIR-ATR没有发现烧结样品发生降解现象的证据，而PCL和共混物的DSC参数显示出变化，这可能是由于在烧结过程中PCL的分子量下降。烧结样品的Cl表明，多糖相均匀分散在PCL基质中，唯一的例外是PCL/D共混物。接触角分析表明，所有样品都是亲水性的。然后将成纤维细胞接种在支架上以评估细胞粘附的速率和程度以及多糖（S、D、G）对基于PCL的共混物的生物活性的影响。
Bioartificial blends of poly-(epsilon-caprolactone) (PCL) with a polysaccharide (starch, S; dextran, D; or gellan, G) (PCL/S, PCL/D, PCL/G 90.9/9.1 wt ratio) were prepared by a solution-precipitation technique and widely characterized by differential scanning calorimetry analysis (DSC), Fourier transform infrared-attenuated total reflectance spectroscopy (FTIR-ATR), optical microscopy (OM), wide-angle X-ray diffraction analysis (WAXD). and thermogravimetry (TGA). DSC showed that the polysaccharide reduced the crystallinity of PCL and had a nucleation effect, which was also confirmed by OM analysis. Hoffman-Weeks analysis was performed on PCL and blend samples allowing calculation of their equilibrium melting temperatures (T-m(0)). WAXD showed that the crystalline unit cell type was the same for PCL and blends. rTIR-ATR did not evidence interactions between blend components. Thermal stability was affected by the type of polysaccharide. Microparticles (< 125 mu m) were produced from blends by cryogenical milling and characterized by scanning electron microscopy analysis (SEM). Selective laser sintering (SLS), a new rapid prototyping technology for scaffold fabrication, was applied to sinter blend microparticles according to a PC-designed two-dimensional geometry (strips and 2 x 2 mm(2), square-meshed grids). The optimal experimental conditions for sintering were established and laser beam parameters (beam speed, BS, and power, P) were found for each blend composition. Morphology of sintered objects was analyzed by SEM and found to be dependent on the morphology of the sintered powders. Sintered samples were analyzed by chemical imaging (CI), FTIR-ATR, DSC, and contact angle analysis. No evidence of the occurrence of degradation phenomena was found by FTIR-ATR for sintered samples, whereas DSC parameters of PCL and blends showed changes which could be attributed to some molecular weight decrease of PCL during sintering. Cl of sintered samples showed that the polysaccharide phase was homogeneously dispersed within the PCL matrix, with the only exception being the PCL/D blend. The contact angle analysis showed that all samples were hydrophilic. Fibroblasts were then seeded on scaffolds to evaluate the rate and the extent of cell adhesion and the effect of the polysaccharides (S, D, G) on the bioactivity of the PCL-based blends.