Cytochrome P450 1A mRNA in the guppy Phalloceros caudimaculatus and response to beta-naphthoflavone and environmental samples.
Cytochrome P450 1A mRNA in the guppy Phalloceros caudimaculatus and response to beta-naphthoflavone and environmental samples.
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DOI：
10.1016/j.aquatox.2016.10.023
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发表时间：
2016-12
影响因子：
4.5
通讯作者：
R. S. Ferreira;C. C. Chivittz-C.;G. B. Santos;J. Zanette
中科院分区：
文献类型：
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作者：
R. S. Ferreira;C. C. Chivittz-C.;G. B. Santos;J. Zanette
细胞色素P450 1A（CYP 1A）mRNA受环境污染物如多环芳烃、多氯联苯和二恶英的诱导。本研究从尾斑孔雀鱼中克隆了CYP 1A转录本，尾斑孔雀鱼代表了南美毒理学研究的潜在鱼类。该基因编码521个氨基酸，与其它鱼类的氨基酸序列同源性为96-70%。在水体暴露于β-萘甲酮（BNF; 1 μM）后，使用RT-qPCR评价了CYP 1A的器官和时间依赖性诱导特征。肝、鳃、肠、脑、臀鳍和鱼种的最短暴露时间为1 h，背鳍为2 h，肾脏和尾鳍为4 h，大多数器官的CYP 1A在实验开始的几个小时（4 h-8 h）内达到诱导峰值，但直到实验结束（96 h）仍保持诱导水平。通过HPLC法对环境样品中CYP 1A的使用进行了验证。caudimaculatusto淘析物来自位于邻近地区的Patos泻湖河口（RS，巴西）的三个溪流的沉积物。肝脏，鳃和尾鳍中的CYP 1A诱导淘析物来自城市（S1）和工业（S2）站点;而不是诱导参考站点位于22公里的潜在污染源，表明环境污染在这种诱导中发挥作用。结果表明，鳍可用于CYP 1A生物标志物分析，并在非致死性活检方法用于环境监测。新发现的CYP 1A对BNF和洗脱液的反应性表明，孔雀鱼P. caudimaculatus可用于南美环境中的环境毒理学研究。
The cytochrome P450 1A (CYP1A) mRNA is induced by environmental contaminants such as PAHs, PCBs and dioxins. The present study cloned theCYP1Atranscript from the guppyPhalloceros caudimaculatus, which represents a potential fish for toxicological studies in South America. The newly identifiedCYP1Aencodes a protein with 521 amino acids that shared 96–70% identity with other fishes. The characterization of organ- and time-dependent induction ofCYP1Ausing RT-qPCR was evaluated after waterborne exposure to beta-naphthoflavone (BNF; 1 μM). The minimum exposure time that elicited significantCYP1Ainduction was 1 h for liver, gill, gut, brain, anal fin and fingerlings; 2 h for dorsal fin; and 4 h for kidney and tail fin.CYP1Atended to reach peak induction in the first few hours (4 h–8 h) of experiment in most organs, although levels remained induced until the end of the experiment (96 h). Validation ofCYP1Ause in environmental sample was performed by exposingP. caudimaculatusto elutriate made from sediment of three streams located in adjacent areas of the Patos Lagoon Estuary (RS, Brazil).CYP1Ain liver, gills and anal fin was induced by elutriate made from urban (S1) and industrial (S2) sites; and not induced by a reference site located 22 Km from potential contaminant sources, suggesting that environmental contamination plays a role in this induction. The results suggest that fins could be used forCYP1Abiomarker analysis and employed in non-lethal biopsy methods for environmental monitoring. The responsiveness of the newly identifiedCYP1Ato BNF and elutriate indicates that the guppyP. caudimaculatuscould be used for environmental toxicology investigations in South American environments.