Low-level predation by lytic phage phiIPLA-RODI promotes biofilm formation and triggers the stringent response in Staphylococcus aureus.
Low-level predation by lytic phage phiIPLA-RODI promotes biofilm formation and triggers the stringent response in Staphylococcus aureus.
复制标题
裂解噬菌体 phiIPLA-RODI 的低水平捕食促进生物膜形成并引发金黄色葡萄球菌的严格反应。
DOI：
10.1038/srep40965
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发表时间：
2017-01-19
影响因子：
4.6
通讯作者：
García P
中科院分区：
文献类型：
--
作者：
Fernández L;González S;Campelo AB;Martínez B;Rodríguez A;García P
从对病原菌的战争中吸取的一个重要教训是需要了解自然微生物群落的生理反应和进化。环境中的细菌群体通常形成受到一定程度噬菌体捕食的生物膜。众所周知，这些多细胞群落对抗菌剂具有抗药性，因此很难根除。这引发了对新的治疗替代方案的研究，包括噬菌体治疗。本研究表明S.在非致死剂量的噬菌体phiIPLA-RODI存在下形成的金黄色葡萄球菌生物膜表现出独特的生理状态，其可能潜在地有益于宿主和捕食者。因此，在噬菌体压力下形成的生物膜更厚并且具有更大的DNA含量。此外，与未处理的对照相比，病毒感染的生物膜显示出主要的转录差异。值得注意的是，RNA-seq数据揭示了严格反应的激活，这可能会减缓噬菌体在生物膜内的前进。最终的结果将是一种平衡，这将有助于细菌细胞经受环境挑战，同时在休眠载体群体重新激活时维持噬菌体可用的敏感细菌细胞库。
An important lesson from the war on pathogenic bacteria has been the need to understand the physiological responses and evolution of natural microbial communities. Bacterial populations in the environment are generally forming biofilms subject to some level of phage predation. These multicellular communities are notoriously resistant to antimicrobials and, consequently, very difficult to eradicate. This has sparked the search for new therapeutic alternatives, including phage therapy. This study demonstrates that S. aureus biofilms formed in the presence of a non-lethal dose of phage phiIPLA-RODI exhibit a unique physiological state that could potentially benefit both the host and the predator. Thus, biofilms formed under phage pressure are thicker and have a greater DNA content. Also, the virus-infected biofilm displayed major transcriptional differences compared to an untreated control. Significantly, RNA-seq data revealed activation of the stringent response, which could slow down the advance of the bacteriophage within the biofilm. The end result would be an equilibrium that would help bacterial cells to withstand environmental challenges, while maintaining a reservoir of sensitive bacterial cells available to the phage upon reactivation of the dormant carrier population.