ASSOCIATION OF THE YEAST PHEROMONE RESPONSE G-PROTEIN BETA-GAMMA-SUBUNITS WITH THE MAP KINASE SCAFFOLD STE5P
ASSOCIATION OF THE YEAST PHEROMONE RESPONSE G-PROTEIN BETA-GAMMA-SUBUNITS WITH THE MAP KINASE SCAFFOLD STE5P
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DOI：
10.1126/science.7667635
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发表时间：
1995-09-15
期刊：
影响因子：
56.9
通讯作者：
LEBERER, E
中科院分区：
文献类型：
--
作者：
WHITEWAY, MS;WU, CL;LEBERER, E
酵母酿酒酵母的交配反应途径包括异源三聚体鸟嘌呤核苷酸结合蛋白（G蛋白），其通过未知机制激活促分裂原活化蛋白（MAP）激酶级联。MAP激酶支架蛋白Ste5p干扰信息素诱导的细胞周期阻滞的氨基末端片段被鉴定。在双杂交试验中还检测到Ste5p的氨基末端和G蛋白β亚基Ste4p之间的单倍体特异性相互作用，并且STE4的信号缺陷等位基因的产物在这种相互作用中是有缺陷的。在具有组成性激活的信息素反应途径的细胞中，表位标记的Ste4p与Ste5p共免疫沉淀，因此，G蛋白和MAP激酶盒通过支架蛋白Ste5p的缔合可以传递G蛋白信号。
The mating response pathway of the yeast Saccharomyces cerevisiae includes a heterotrimeric guanine nucleotide-binding protein (G protein) that activates a mitogen-activated protein (MAP) kinase cascade by an unknown mechanism. An amino-terminal fragment of the MAP kinase scaffold protein Ste5p that interfered with pheromone-induced cell cycle arrest was identified. A haploid-specific interaction between the amino terminus of Ste5p and the G protein beta subunit Ste4p was also detected in a two-hybrid assay, and the product of a signaling-defective allele of STE4 was defective in this interaction. In cells with a constitutively activated pheromone response pathway, epitope-tagged Ste4p was coimmunoprecipitated with Ste5p, Thus, association of the G protein and the MAP kinase cassette via the scaffolding protein Ste5p may transmit the G protein signal.