Isolation of Burkholderia jumbo phages and their utilization as biocontrol agents to suppress rice seedling rot disease
Isolation of Burkholderia jumbo phages and their utilization as biocontrol agents to suppress rice seedling rot disease
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DOI：
10.1007/s10327-022-01107-z
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发表时间：
2022-11
影响因子：
1.2
通讯作者：
Ayane Kanaizuka;Ryota Sasaki;S. Miyashita;S. Ando;Kumiko Ito;T. Fukuhara;Hideki Takahashi
中科院分区：
文献类型：
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作者：
Ayane Kanaizuka;Ryota Sasaki;S. Miyashita;S. Ando;Kumiko Ito;T. Fukuhara;Hideki Takahashi
巨型噬菌体的基因组DNA大于200kbp，由于与普通噬菌体相比，它们通常具有更广泛的植物病原菌寄主范围，因此作为潜在的生防剂来抑制栽培作物中的细菌病害正受到人们的关注。因此，需要从环境和农业资源中识别新的巨噬菌体，以开发有效的生物防治材料。在针对植物病原菌Burkholderia glumae的噬菌体调查中，从落叶堆肥中分离到FLC8、FLC9和FLC10噬菌体。这三个噬菌体都具有二十面体的头部和尾部结构。FLC8和FLC9的基因组DNA分别为225、545和321,833个核苷酸，可归类为巨噬菌体，而FLC10的基因组DNA为32,867个核苷酸，表明它是一个普通噬菌体。三种噬菌体均能明显抑制BYB引起的水稻秧苗腐烂病。Glumae：FLC8为77.1，FLC9为82.1，FLC10为33.3。FLC8和FLC9可侵染5B。Glumae和SixB。通过噬菌体寄主范围分析，均为Plantariistrains菌株，而FLC10的寄主范围较窄。对两个巨噬菌体保存条件的评价表明，在4℃的噬菌体缓冲液(pH 7.5)中，两个巨噬菌体的 ~ 感染力可保持90%的 ~ 30d。
Jumbo bacteriophages, which have genomic DNA larger than 200 k-bp, are attracting attention as potential biocontrol agents to suppress bacterial diseases in cultivated crops because they generally have a broader host range of phytopathogenic bacteria compared with ordinary bacteriophages. Thus, the identification of new jumbo phages from environmental and agricultural resources is required to develop effective biological control materials. In a survey of phages targeting the phytopathogenic bacteriumBurkholderia glumae, bacteriophages FLC8, FLC9, and FLC10 were isolated from fallen leaf compost. All three phages had an icosahedral head and tail structure. FLC8 and FLC9 had 225,545-bp and 321,833-bp genomic DNA, respectively, indicating that they could be classified as jumbo phages, whereas FLC10 had 32,867-bp genomic DNA, indicating that it was an ordinary phage. All three phages clearly suppressed rice seedling rot disease caused byB. glumae: the control values were 77.1 of FLC8, 82.1 of FLC9 and 33.3 of FLC10, respectively. FLC8 and FLC9 could infect fiveB. glumaeand sixB. plantariistrains, which were all strains using analysis of phage host range, while FLC10 had a narrow host range. Evaluation of the preservation condition of both jumbo phages revealed that ~ 90% infectivity of them was maintained in phage buffer (pH 7.5) at 4 °C for ~ 30 days.