Dynamics of vegetative cytoplasm during generative cell formation and pollen maturation in Arabidopsis thaliana
Dynamics of vegetative cytoplasm during generative cell formation and pollen maturation in Arabidopsis thaliana
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DOI：
10.1007/bf01273170
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发表时间：
1996-01-01
期刊：
影响因子：
2.9
通讯作者：
Musgrave, ME
中科院分区：
文献类型：
--
作者：
Kuang, A;Musgrave, ME
研究了拟南芥生殖细胞形成和花粉成熟过程中花粉细胞质的超微结构变化。花粉细胞质发育成复杂的超微结构，并在这些时期发生剧烈变化。生殖细胞形成后，脂滴增多，其组织和分布随发育阶段而变化。淀粉体中的淀粉粒在生殖和精子形成过程中数量和大小增加，在花粉成熟时减少。线粒体的形态和膜系统变化不大。网囊变得非常突出，在精子形成过程中和之后可以观察到许多相关的小泡。在精子形成过程中，内质网广泛地以堆叠的形式出现。游离核糖体和多聚核糖体在所有发育阶段的细胞质中都很丰富，尽管它们在某些阶段和某些区域表现得更密集。在成熟花粉中，所有细胞器随机分布在营养细胞质中，并出现大量小颗粒。讨论了储藏物质的组织和分布，以及这些小颗粒在生殖、精子形成和花粉成熟过程中的出现。
Ultrastructural changes of pollen cytoplasm during generative cell formation and pollen maturation in Arabidopsis thaliana were studied. The pollen cytoplasm develops a complicated ultrastructure and changes dramatically during these stages. Lipid droplets increase after generative cell formation and their organization and distribution change with the developmental stage. Starch grains in amyloplasts increase in number and size during generative and sperm cell formation and decrease at pollen maturity. The shape and membrane system of mitochondria change only slightly. Dictyosomes become very prominent, and numerous associated vesicles are observed during and after sperm cell formation. Endoplasmic reticulum appears extensively as stacks during sperm cell formation. Free and polyribosomes are abundant in the cytoplasm at all developmental stages although they appear denser at certain stages and in some areas. In mature pollen, all organelles are randomly distributed throughout the vegetative cytoplasm and numerous small particles appear. Organization and distribution of storage substances and appearance of these small particles during generative and sperm cell formation and pollen maturation are discussed.