Seasonal changes in the feeding pattern of Culex pipiens pallens govern the transmission dynamics of multiple lineages of avian malaria parasites in Japanese wild bird community
Seasonal changes in the feeding pattern of Culex pipiens pallens govern the transmission dynamics of multiple lineages of avian malaria parasites in Japanese wild bird community
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DOI：
10.1111/j.1365-294x.2010.04897.x
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发表时间：
2010-12-01
影响因子：
4.9
通讯作者：
Tsuda, Y.
中科院分区：
文献类型：
--
作者：
Kim, K. S.;Tsuda, Y.
蚊媒病原体传播的异质性主要取决于野生动物种群中蚊虫叮咬的分布模式。尽管蚊子对禽疟寄生虫的传播至关重要，但人们对自然媒介的生态知之甚少。本研究采用聚合酶链反应（PCR）方法检测淡色库蚊的血食和寄生虫发病率，以确定蚊子的摄食方式如何影响日本野鸟中禽疟寄生虫的传播动态。我们收集了881例未喂养的Cx和486例血液喂养的Cx。在东京的一个公园里，淡色库蚊在植被上休息。分别取腹、胸两组进行PCR筛选。将未喂食蚊子的腹部组合成95个池。从中，我们扩增了32个（33.7%）池的疟原虫DNA。在血供蚊中，对371只蚊进行了血源和疟原虫筛查。疟原虫DNA从以13种鸟类中的6种为食的蚊子中扩增出来。基于细胞色素b基因478 bp，鉴定出10个疟原虫世系，序列差异为0.2 ~ 10%。在蚊子腹部和胸部均检测到3种最常见的疟原虫谱系（CXPIP09、SGS1和PADOM02），强烈提示这些疟原虫谱系存在传播。丛林乌鸦（Corvus macrohynchos）是三种最常见疟原虫的天然宿主，占血液来源的63.8%。由于丛林乌鸦的蚊媒摄食量显著增加，适值丛林乌鸦的繁殖季节，本研究认为丛林乌鸦是疟原虫传播的主要宿主。
Heterogeneity in the transmission of mosquito-borne pathogens is determined largely by distribution patterns of mosquito bites among wild animal populations. Although mosquitoes are crucial for transmission of avian malaria parasites, little is known about the ecology of natural vectors. We examined bloodmeal and parasite incidence in Culex pipiens pallens by a polymerase chain reaction (PCR)-based procedure to determine how the feeding pattern of mosquitoes govern transmission dynamics of avian malaria parasites in Japanese wild birds. We collected 881 unfed and 486 blood-fed Cx. pipiens pallens resting on vegetation in a park in Tokyo. The mosquitoes were separated into abdomen and thorax prior to PCR screening. Abdomens of unfed mosquitoes were combined into 95 pools. From these, we amplified Plasmodium DNA in 32 (33.7%) pools. Among blood-fed mosquitoes, 371 individuals were screened for blood-sources and Plasmodium parasites. Plasmodium DNA was amplified from mosquitoes fed on 6 of 13 avian species identified as blood-sources. Ten Plasmodium lineages were identified on the basis of 478 bp of the cytochrome b gene, with 0.2-10% sequence divergence. The three commonest Plasmodium lineages (CXPIP09, SGS1 and PADOM02) were detected in both the abdomens and thoraxes of mosquitoes, strongly suggesting transmission of these lineages. Jungle crow (Corvus macrorhynchos) served as a natural host for the three commonest Plasmodium lineages and made up 63.8% of blood-sources. As a significant increase in feeding of vector mosquitoes on jungle crows coincided with their breeding season, jungle crows were considered to be the primary reservoir of Plasmodium transmission in this study.