The Sko1 protein represses the yeast-to-hypha transition and regulates the oxidative stress response in Candida albicans
The Sko1 protein represses the yeast-to-hypha transition and regulates the oxidative stress response in Candida albicans
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DOI：
10.1016/j.fgb.2010.03.009
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发表时间：
2010-07-01
影响因子：
3
通讯作者：
Pla, Jesus
中科院分区：
文献类型：
--
作者：
Alonso-Monge, Rebeca;Roman, Elvira;Pla, Jesus
细胞对环境变化的反应触发适应性反应，其部分由转录反应介导。这些反应是复杂的，并且依赖于不同的转录因子。目前的工作报告的Sko 1蛋白的含义在几个过程中相关的生理学的白色念珠菌。首先，Sko 1作为参与发病机制和菌丝形成的基因的转录抑制因子，这导致菌丝相关基因ECE 1和HWP 1的表达增加，而使用小鼠全身感染模型的毒力没有显著变化。第二，Sko 1参与氧化应激反应，并且sko 1突变体增加hog 1对髓单核细胞系HL-60的敏感性。过氧化氢处理后的全基因组转录分析表明，sko 1突变体能够产生类似于野生型菌株的适应性反应，虽然重要的差异检测到的转录反应的幅度。总的来说，这些结果暗示Sko 1作为C.白色念珠菌(C)2010年爱思唯尔公司All rights reserved.
Cells respond to environmental changes triggering adaptive responses which are, in part, mediated by a transcriptional response. These responses are complex and are dependent on different transcription factors. The present work reports the implication of the Sko1 protein in several processes relevant to the physiology of Candida albicans. First, Sko1 acts as transcriptional repressor of genes involved in pathogenesis and hyphal formation, which results in increased expression of the hyphal related genes ECE1 and HWP1 without significant changes in the virulence using a mouse model of systemic infection. Second Sko1 is involved in the response to oxidative stress and sko1 mutants increase the sensitivity of hog1 to the myelomonocytic cell line HL-60. Genome-wide transcriptional analysis after hydrogen peroxide treatment revealed that sko1 mutants were able to generate an adaptive response similar to wild type strains, although important differences were detected in the magnitude of the transcriptional response. Collectively, these results implicate Sko1 as an important mediator of the oxidative stress response in C. albicans. (C) 2010 Elsevier Inc. All rights reserved.