Developmental consequences of the tumorous shoot development1 mutation, a novel allele of the cellulose-synthesizing KORRIGAN1 gene
Developmental consequences of the tumorous shoot development1 mutation, a novel allele of the cellulose-synthesizing KORRIGAN1 gene
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DOI：
10.1007/s11103-009-9546-2
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发表时间：
2009-12-01
影响因子：
5.1
通讯作者：
Schmuelling, Thomas
中科院分区：
文献类型：
--
作者：
Krupkova, Eva;Schmuelling, Thomas
这项工作描述了进一步表征的tumorous芽发育1（tsd 1）突变体的拟南芥，它开发了无组织的肿瘤样芽组织，而不是有组织的叶和茎。基于图位的克隆显示，tsd 1是KOR 1基因的一个新的强等位基因，编码一种参与纤维素合成的膜结合内切-1，4-β-D-葡聚糖酶。为了研究伴随着tsd 1突变体异常生长的发育变化，通过标记基因分析了分生组织中的图案和激素状态。分生组织维持的关键调节因子CLV 3和STM基因的表达表明，在tsd 1芽愈伤组织中存在许多分生组织。LFY：：GUS标记的表达支持了tsd 1愈伤组织形成器官原基的能力，然而其未能进一步发育。一个表皮标记表明，L1层只保持在不同地区的tsd 1愈伤组织，这可能是一个原因，无组织的芽生长。在tsd 1根分生组织中，萌发后早期静止中心活性丧失，导致根分生组织分化。在tsd 1突变体中，报告生长素和细胞分裂素状态的基因的空间表达被改变。修改这些激素的内源水平部分挽救了tsd 1突变体的芽和根的发育。总之，这项工作表明，TSD 1/KOR 1是维持正确的分生组织模式和器官生长以及正常激素反应所必需的。
This work describes the further characterization of the tumorous shoot development1 (tsd1) mutant of Arabidopsis thaliana, which develops disorganized tumorous-like shoot tissue instead of organized leaves and stems. Map-based cloning revealed that tsd1 is a novel strong allele of the KOR1 gene, encoding a membrane-bound endo-1,4-beta-d-glucanase involved in cellulose synthesis. To study developmental changes accompanying the aberrant growth of the tsd1 mutant, patterning in the meristems and the hormonal status were analysed by marker genes. Expression of key regulators of meristem maintenance, the CLV3 and STM genes, indicated the presence of numerous meristems in the tsd1 shoot callus. Expression of the LFY::GUS marker supported the ability of the tsd1 callus to form organ primordia, which however failed to develop further. An epidermal marker showed that the L1 layer was maintained only in distinct areas of the tsd1 callus, which could be a reason of disorganized shoot growth. In the tsd1 root meristem, quiescent center activity was lost early after germination, which caused differentiation of the root meristem. The spatial expression of genes reporting the auxin and cytokinin status was altered in the tsd1 mutant. Modifying the endogenous levels of these hormones partially rescued shoot and root development of the tsd1 mutant. Together, the work shows that TSD1/KOR1 is required for maintaining a correct meristematic pattern and organ growth as well as for a normal hormonal response.