Molecular cloning, characterisation and tissue-specific expression of human LAG3, a member of the novel Lag1 protein family
Molecular cloning, characterisation and tissue-specific expression of human LAG3, a member of the novel Lag1 protein family
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DOI：
10.1080/1042517021000041831
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发表时间：
2003-04-01
期刊：
影响因子：
--
通讯作者：
Gong, Y
中科院分区：
文献类型：
--
作者：
Cai, XF;Tao, Z;Gong, Y
我们鉴定并鉴定了人LAG3的编码基因，它是与酿酒酵母长寿保障基因LAG1相关的一个新的真核拉丁蛋白家族成员。该复合核苷酸序列包括1140个核苷酸编码区、210个碱基的5‘-非编码区和900个碱基对的3’-非编码区。推导的380个氨基酸序列有6个跨膜区和保守的Lag1基序。Northern印迹分析表明，LAG3Hs转录本在所有12个人体组织中都有表达，在肾脏和肝脏中表达较高，在脑、心脏、骨骼肌、胎盘和肺中表达中等，在结肠、胸腺、脾、小肠和外周血白细胞中表达较低。由于其结构上与LAG1相似，推测LAG3Hs可能参与神经鞘磷脂的代谢。
We identified and characterised the cDNA coding for human Lag3, a member of the novel eukaryotic Lag1 protein family which was related to the Saccharomyces cerevisiae longevity-assurance gene LAG1. The composite nucleotide sequence revealed a coding region of 1140 bp, a 5'-UTR of 210 by and a 3'-UTR of 900 bp. The deduced sequence of 380 amino acids had six transmembrane regions and the conserved Lag1 motif. Northern blot analysis revealed that the similar to2.2 kb LAG3Hs transcript was expressed in all 12 human tissues, high in kidney and liver, moderate in brain, heart, skeletal muscle, placenta and lung and low in colon, thymus, spleen, small intestine and peripheral blood leukocyte. Because of its structural similarity to LAG1, it eras postulated that LAG3Hs may mediate sphingolipiids metabolism.