DISTINCT SPATIAL LOCALIZATION OF SPECIFIC MESSENGER-RNAS IN CULTURED SYMPATHETIC NEURONS
DISTINCT SPATIAL LOCALIZATION OF SPECIFIC MESSENGER-RNAS IN CULTURED SYMPATHETIC NEURONS
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DOI：
10.1016/0896-6273(90)90340-l
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发表时间：
1990-12-01
期刊：
影响因子：
16.2
通讯作者：
FREMEAU, RT
中科院分区：
文献类型：
--
作者：
BRUCKENSTEIN, DA;LEIN, PJ;FREMEAU, RT
我们在培养的交感神经元中检测了特定mrna的亚细胞分布。在适当的条件下，交感神经元延伸轴突和树突，这是通过光镜和免疫细胞化学标准区分的。原位杂交显示树突内mRNA的差异定位。编码MAP2的mRNA在细胞体中丰富，在树突间室中分布不均匀，但在轴突中未检测到。相比之下，编码GAP-43和α -微管蛋白的mrna仅限于细胞体，在很大程度上被排除在树突和轴突之外。洗涤剂提取显示，大多数与树突相关的mRNA编码MAP2与细胞的Triton X-100不溶性部分相关。存在于树突腔室的mrna子集可能编码参与树突形态发生和重塑的蛋白质。
We examined the subcellular distribution of specific mRNAs in cultured sympathetic neurons. Under appropriate conditions, sympathetic neurons extend both axons and dendrites that are distinguishable by light microscopic and immunocytochemical criteria. In situ hybridization revealed a differential localization of mRNA within dendrites. mRNA encoding MAP2 was abundant in cell bodies and distributed nonhomogeneously throughout the dendritic compartment, but was not detected in axons. In contrast, mRNAs encoding GAP-43 and alpha-tubulin were restricted to the cell body and largely excluded from dendrites as well as axons. Detergent extraction revealed that most dendrite-associated mRNA encoding MAP2 was associated with the Triton X-100 insoluble fraction of the cell. The subset of mRNAs present in the dendritic compartment may encode proteins involved in the morphogenesis and remodeling of dendrites.