Screening of 42 medicinal plants for in vivo anthelmintic activity against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus)
Screening of 42 medicinal plants for in vivo anthelmintic activity against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus)
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筛选 42 种药用植物对金鱼 (Carassius auratus) 体内指环虫 (Monogenea) 的驱虫活性
DOI：
10.1007/s00436-011-2805-6
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发表时间：
2012-07-01
影响因子：
2
通讯作者：
Chen, Peng
中科院分区：
文献类型：
--
作者：
Ji, Jie;Lu, Cheng;Chen, Peng
研究了42种传统药用植物甲醇提取物对金鱼（Carassius auratus）中阔叶草（Dactylogyrus mongenea）有较强的驱虫活性。肉桂（Cinnamomum cassia）、林芝（Lindera aggregata）和山柰（pseudoolarix kaempferi）均表现出100%的活性，分别采用石油醚、氯仿、乙酸乙酯、甲醇和水5种溶剂提取样品，并进行体内生物测定。其中，桂花水提物和甲醇提物的EC（50）值最高，分别为13.2和12.3 mg L(-1)，对中间蚜虫的EC（50）值为30.0和40.0 mg L(-1)， 100%有效；其次是桂花甲醇提物，暴露48 h后EC（50）值为17.1 mg L(-1), 60.0 mg L(-1)， 100%有效。山奈草甲醇和乙酸乙酯提取物在60.0 mg L（-1）和50.0 mg L（-1）时对中间田鼠的防治效果为100%，EC（50）值分别为23.5 mg L和23.3 mg L（-1）时的防治效果相似。研究了这些活性提取物对金鱼48 h的急性毒性，相应的中位致死浓度（LC(50)）分别为56.9、31.3、88.7、168.2和165.7 mg L（-1）。这些结果表明，这三种植物的提取物可以开发为治疗中间曲霉的首选天然抗寄生虫剂。
In the present study, methanol extracts of 42 traditional medicinal plants with potent anthelmintic activity against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus) were investigated. Cinnamomum cassia, Lindera aggregata, and Pseudolarix kaempferi exhibited 100% activity and were selected for further evaluation by applying five solvents (petroleum ether, chloroform, ethyl acetate, methanol, and water) for the extraction of the samples, followed by the in vivo bioassay. Among the extracts tested, water and methanol extracts of C. cassia showed the highest efficacies with EC(50) values of 13.2 and 12.3 mg L(-1), showing 100% efficacy against D. intermedius at 30.0 and 40.0 mg L(-1), followed by methanol extract of L. aggregata which demonstrated 100% efficacy at 60.0 mg L(-1) with EC(50) value of 17.1 mg L(-1) after 48 h of exposure. Methanol and ethyl acetate extract of P. kaempferi, which exhibited a 100% efficacy against D. intermedius at 60.0 and 50.0 mg L(-1), revealed similar activity with EC(50) values of 23.5 and 23.3 mg L(-1), respectively. Acute toxicity of these active extracts was investigated on goldfish for 48 h and the corresponding median lethal concentrations (LC(50)) of 56.9, 31.3, 88.7, 168.2, and 165.7 mg L(-1), respectively. These findings indicated that these extracts of the three plants can be developed as preferred natural antiparasitic agents for the treatment of D. intermedius.