REACTIVE HYPEREMIA IN INDIVIDUAL CAPILLARIES OF SKELETAL-MUSCLE
REACTIVE HYPEREMIA IN INDIVIDUAL CAPILLARIES OF SKELETAL-MUSCLE
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DOI：
10.1152/ajplegacy.1972.223.3.517
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发表时间：
1972-01-01
影响因子：
--
通讯作者：
JOHNSON, PC
中科院分区：
文献类型：
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作者：
BURTON, KS;JOHNSON, PC
方法体重1.5- 2.6kg的猫，用1 mg/kg盐酸丙丙嗪镇静，用75 mg/kg α-氯蔗糖麻醉。从左后肢手术切除缝匠肌，注意尽可能多地保留从股动脉和静脉到肌肉的交通血管。在整个解剖过程中，用林格液保持肌肉湿润。分离后，如图1所示，通过1.6 mm内径的聚乙烯管，用右后肢股动脉的血液连续灌注肌肉。通过斯坦森压力传感器在该回路中监测动脉血压。静脉流出物通过液滴计数器监测总流速，然后通过颈外静脉将血液返回动物体内。通过连接到肌肉和液滴计数器之间的静脉回路的斯坦森压力传感器测量静脉压力。在整个实验过程中，静脉压保持在约14 mm Hg。我是说。为了确保良好的透照肌肉，有必要从肌肉表面去除尽可能多的筋膜。这是用烧灼器完成的，小心避免在该过程中加热或干燥肌肉。然后将肌肉固定在框架上并置于保持在37 ℃的加热的显微镜载物台上。用林格氏溶液浸泡肌肉，并用萨兰包裹物覆盖以防止实验期间干燥。在这些实验中，肌肉重量平均为4.1 g。使用Wayland和约翰逊（21）描述的双缝光度法测量毛细血管中红细胞的速度。用汞弧灯照射安装在显微镜载物台上的肌肉，并将毛细血管的放大图像投影到显微镜上。
METHODSCats in a mass range of 1.5-2.6 kg were tranquilized with 1 mg/kg propiopromazine hydrochloride and anesthetized with 75 mg/kg of a-chlorolose. The sartorius muscle was surgically removed from the left hindlimb, with care being taken to preserve as many of the communicating blood vessels from the femoral artery and vein to the muscle as possible. The muscle was kept moist with Ringer solution throughout the dissection procedure. After isolation, the muscle was continuously perfused with blood from the femoral artery in the right hindlimb through 1.6 mm id polyethylene tubing as shown in Fig. 1. Arterial blood pressure was monitored in this circuit by a Statham pressure transducer. Venous outflow passed through a drop counter to monitor the gross flow rate, and the blood was then returned to the animal through the external jugular vein. Venous pressure was measured by a Statham pressure transducer connected to the venous circuit between the muscle and the drop counter. The venous pressure was maintained at about 14 mm Hg throughout the experi. ment. To insure good transillumination of the muscle, it was necessary to remove as much fascia from the surface of the muscle as possible. This was done with a cautery, with care taken to avoid heating or drying of the muscle during this procedure. The muscle was then mounted on a frame and placed on a heated microscope stage maintained at 37 C. The muscle was bathed with Ringer solution and covered with Saran wrap to prevent drying during the experiment. Muscle weight averaged 4.1 g in these experiments. The velocities of red cells in the capillaries were measured using the dual-slit photometric method described by Wayland and Johnson (21). The muscle mounted on the microscope stage was illuminated with a mercury-arc lamp, and the magnified image of the capillaries was projected onto a