Conformational study of N(epsilon)-(carboxymethyl)lysine adducts of recombinant alpha-crystallins.
Conformational study of N(epsilon)-(carboxymethyl)lysine adducts of recombinant alpha-crystallins.
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重组α-晶状体蛋白的N(ε)-(羧甲基)赖氨酸加合物的构象研究。
DOI：
10.1076/ceyr.18.4.270.5364
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发表时间：
1999
影响因子：
2
通讯作者：
Liang,JJ
中科院分区：
文献类型：
--
作者：
Akhtar,NJ;Sun,TX;Liang,JJ
PurposeLens proteins underwent nonenzymatic glycation, and the advanced glycation end products (AGEs) were detected by immunological assays. One of the major AGE structures is N?-(carboxymethyl)lysine (CML). Since the involvement of AGEs in the pathogenesis of diabetic complications is speculated, the effects of CML formation on proteins were studied.MethodsCML adducts were generated in recombinant aA-and aB-crystallins by incubation with glyoxylic acid and NaBH 3 CN. SDS-PAGE and size exclusion chromatography were used to detect subunit degradation and high-molecular-weight (HMW) aggregation. Conformational change was determined by fluorescence and circular dichroism (CD) measurements. The chaperone function was studied by DTT-induced aggregation of insulin.ResultsLysine modification was estimated to be 60–90% depending on the conditions of incubation. No subunit degradation or HMW aggregation was observed. Fluorescence and CD measurements detected a conformational change in CML adducts. Measurements of chaperone-like activity, however, indicated that the formation of CML increased the protein's ability to protect insulin against DTT-induced aggregation.ConclusionsAlthough CML adducts of aA- and aB-crystallins, the major AGE structures formed in vitro, changed protein conformation, no subunit degradation and HMW aggregation were observed. Moreover, the CML adducts increased chaperone-like activity of both aA- and aB-crystallins. The results suggest that CML formation alone may not play a major role in protein aggregation and lens opacity.
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探索牛 α-乳清蛋白的不同构象状态：4,4-双[1-(苯基氨基)-8-萘磺酸盐]的荧光研究。
影响因子：
2.9
作者：
Musci,G;Berliner,LJ
通讯作者：
Berliner,LJ
一些翻译后修饰对α-晶状体蛋白的分子伴侣样活性的影响。
影响因子：
2.1
作者：
M. V. van Boekel;S. Hoogakker;J. Harding;W. D. de Jong
通讯作者：
W. D. de Jong
影响因子：
2.9
作者：
REDDY, S;BICHLER, J;BAYNES, JW
通讯作者：
BAYNES, JW
DOI：
10.1056/nejm198805193182007
发表时间：
1988-05
期刊：
The New England journal of medicine
影响因子：
--
作者：
M. Brownlee;A. Cerami;H. Vlassara
通讯作者：
M. Brownlee;A. Cerami;H. Vlassara
影响因子：
3.5
作者：
DAS, KP;SUREWICZ, WK
通讯作者：
SUREWICZ, WK