Porcine Cysticercosis: Possible Cross-Reactivity of Taenia hydatigena to GP50 Antigen in the Enzyme-Linked Immunoelectrotransfer Blot Assay
Porcine Cysticercosis: Possible Cross-Reactivity of Taenia hydatigena to GP50 Antigen in the Enzyme-Linked Immunoelectrotransfer Blot Assay
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DOI：
10.4269/ajtmh.17-0378
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发表时间：
2017-01-01
影响因子：
3.3
通讯作者：
O'Neal, Seth E.
中科院分区：
文献类型：
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作者：
Muro, Claudio;Gomez-Puerta, Luis A.;O'Neal, Seth E.
小扁豆凝集素糖蛋白酶联免疫电转移印迹（LLGP EITB，据报道灵敏度99%，特异性100%）被用作猪猪带绦虫暴露的血清学标记物。然而，只有有限数量的寄生虫被评估为交叉反应性。猪可能携带其他相关的寄生虫感染，包括带菌带绦虫，尚未正式评估其交叉反应。我们调查了秘鲁Tumbes的一个畜栏，该地区于2012年完成了囊虫病消除示范项目。在该畜栏中，14/19(73.7%)6-8周龄仔猪对LLGP EITB的GP50有反应，且均有循环带绦虫抗原。来自8头死亡仔猪；其中4例感染了棘球绦虫，而没有一例感染了棘球绦虫。随后，两只居民犬被确认患有带绦虫病。这些结果表明，猪体内存在GP50交叉反应性，但还需要对照实验感染来证实这一假设。
The lentil lectin glycoprotein enzyme-linked immunoelectrotransfer blot (LLGP EITB, reported sensitivity 99% and specificity 100%) is used as a serologic marker of exposure to Taenia solium in pigs. However, only a limited number of parasites have been evaluated for cross reactivity. Pigs may host other related cestode infections, including Taenia hydatigena, which have not been formally evaluated for cross-reactions. We investigated a corral in Tumbes, Peru, a region where a cysticercosis elimination demonstration project was completed in 2012. In this corral, 14/19 (73.7%) 6-8-week-old piglets were reactive to GP50 on LLGP EITB, and all had circulating Taenia sp. antigens. From eight necropsied piglets; four were infected with T. hydatigena metacestodes whereas none had evidence of T. solium infection. Two resident dogs were subsequently confirmed to have T. hydatigena taeniasis. These results suggest GP50 cross-reactivity in T. hydatigena-infected pigs, although controlled experimental infection is needed to confirm this hypothesis.