Strain increases airway smooth muscle cell proliferation.
Strain increases airway smooth muscle cell proliferation.
复制标题
压力会增加气道平滑肌细胞的增殖。
DOI：
10.1165/ajrcmb.10.1.8292386
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发表时间：
1994
影响因子：
6.4
通讯作者：
Bruce,MC
中科院分区：
文献类型：
--
作者：
Smith,PG;Janiga,KE;Bruce,MC
气道平滑肌（ASM）细胞增生是肺部疾病的常见现象，在这种疾病中，增加跨肺压是充分通气所必需的。 ASM 细胞的机械变形对观察到的增生性变化的影响程度尚未得到解决。为了确定体外循环拉伸是否会改变 ASM 细胞增殖和蛋白质含量，将犬 ASM 细胞培养在胶原涂层硅橡胶膜上，进行拉伸松弛方案，并与摇动平台上培养的细胞或对照（未操作）细胞进行比较。在细胞生长的对数期，循环拉伸导致细胞数量增加（P < 0.001）。在拉伸、摇动和对照细胞中，在培养 5 或 14 天后，在 12 小时脉冲期间评估 [3H] 胸苷 ([3H]TdR) 的掺入。与摇动细胞或对照细胞相比，在第 5 天而非第 14 天时，拉伸细胞中每个细胞的 [3H]TdR 掺入有所增加。细胞达到汇合后测定的每孔总细胞蛋白含量在拉伸细胞中高于对照（拉伸细胞为 74.2 +/- 7.9，对照为 49.1 +/- 5.6，对照为 51.1 +/- 6.5；微克/10(5) 细胞；P < 0.05）。拉伸细胞的透射电子显微镜观察表明，与对照细胞相比，肌丝浓度增加。这些观察结果表明，拉伸可能会导致 ASM 增生和气道阻力增加。
Airway smooth muscle (ASM) cell hyperplasia is a common finding in lung diseases in which increased transpulmonary pressure is necessary for adequate ventilation. The extent to which mechanical deformation of ASM cells contributes to the observed hyperplastic changes has not been addressed. To determine if cyclic stretch alters ASM cell proliferation and protein content in vitro, canine ASM cells were cultured on collagen-coated silastic membranes, subjected to a stretch-relaxation regimen, and compared with cultured cells on a rocking platform or with control (unmanipulated) cells. During the log phase of cell growth, cyclic stretch resulted in increased cell numbers (P < 0.001). Incorporation of [3H]thymidine ([3H]TdR) was assessed after 5 or 14 days in culture during 12-h pulse in stretched, rocked, and control cells. Incorporation of [3H]TdR per cell was elevated in stretched cells when compared with either rocked or control cells at 5 days but not 14 days. Total cellular protein content per well determined after cells had reached confluence was greater in stretched cells than in the controls (74.2 +/- 7.9 stretched versus 49.1 +/- 5.6 control versus 51.1 +/- 6.5 rocker; microgram/10(5) cells; P < 0.05). Transmission electron microscopic observations of stretched cells suggested increased concentrations of myofilaments compared with control cells. These observations suggest that stretch may contribute to increased ASM hyperplasia and airway resistance.