Interleukin-4 and interleukin-5 map to human chromosome 5 in a region encoding growth factors and receptors and are deleted in myeloid leukemias with a del(5q).
Interleukin-4 and interleukin-5 map to human chromosome 5 in a region encoding growth factors and receptors and are deleted in myeloid leukemias with a del(5q).
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DOI：
10.1182/blood.v73.3.647.bloodjournal733647
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发表时间：
1989-02
期刊：
影响因子：
20.3
通讯作者：
M. Beau;Richard S. Lemons;R. Espinosa;Richard A. Larson;N. Arai;Janet D. Rowley
中科院分区：
文献类型：
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作者：
M. Beau;Richard S. Lemons;R. Espinosa;Richard A. Larson;N. Arai;Janet D. Rowley
白细胞介素-4（IL-4）是几种造血谱系细胞生长和分化的有效介质。白细胞介素-5（IL-5）是一种谱系特异性造血生长因子，可刺激正常人骨髓细胞产生嗜酸性粒细胞和嗜酸性粒细胞集落。通过使用体细胞杂交和原位染色体杂交，我们将IL-4和IL-5基因定位于人5号染色体q23-31带，这是一个在髓系疾病患者中经常缺失[del（5 q）]的染色体区域。应用原位杂交技术，在4例5 q缺失的难治性贫血（RA）或治疗相关性急性非淋巴细胞白血病（t-ANLL）患者的5 q染色体上发现IL-4和IL-5基因缺失。因此，5号染色体的一小段含有IL-4、IL-5、IL-3和GM-CSF以及其他基因如CD 14和EGR 1。我们发现这些基因中的每一个都在5 q染色体上缺失，这表明这些基因中的一个或多个的功能丧失可能在与del（5 q）相关的血液病的发病机制中起重要作用。
Interleukin-4 (IL-4) is a potent mediator of growth and differentiation of cells of several hematopoietic lineages. Interleukin-5 (IL-5) is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. By using somatic cell hybrids and in situ chromosomal hybridization, we localized the IL-4 and IL-5 genes to human chromosome 5 at bands q23-31, a chromosomal region that is frequently deleted [del(5q)] in patients with myeloid disorders. By in situ hybridization, the IL-4 and IL-5 genes were found to be deleted in the 5q- chromosome of four patients with refractory anemia (RA) or therapy-related acute nonlymphocytic leukemia (t-ANLL), who had a del(5q). Thus a small segment of chromosome 5 contains IL-4, IL-5, IL-3, and GM-CSF as well as other genes such as CD14 and EGR1. Our findings that each of these genes was deleted in the 5q- chromosome suggest that loss of function of one or more of these genes may play an important role in the pathogenesis of hematologic disorders associated with a del(5q).