Comparison of the CLSI Guideline and ISO/IDF Standard for Antimicrobial Susceptibility Testing of Lactobacilli
Comparison of the CLSI Guideline and ISO/IDF Standard for Antimicrobial Susceptibility Testing of Lactobacilli
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DOI：
10.1089/mdr.2013.0189
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发表时间：
2014-12-01
影响因子：
2.6
通讯作者：
Domig, Konrad J.
中科院分区：
文献类型：
--
作者：
Mayrhofer, Sigrid;Zitz, Ulrike;Domig, Konrad J.
乳酸杆菌作为益生菌和发酵剂在发酵食品的生产中起着至关重要的作用。虽然乳酸杆菌是一个技术上有用和有益的细菌群，但其中一些成员很少与细菌感染相关。相应地，临床医生对乳杆菌的抗菌药物敏感性很感兴趣。此外，抗菌药物敏感性测试（AST）也与商业应用的乳酸杆菌有关，因为携带可转移抗生素抗性基因的细菌菌株不应用于发酵和益生菌食品。因此，不同组织开发了两种方法，并在本研究中进行了比较。为此，根据临床和实验室标准化研究所（CLSI）和国际标准化组织（ISO）/国际乳品联合会（IDF）的程序，对22株乳杆菌型菌株进行了16种抗生素的抗菌药物敏感性试验。检测到两种方法之间的重大差异主要是由于AST培养基不同。因此，半数供试菌株在CLSI培养基中不能持续生长，而所有菌株在ISO/IDF培养基中均显示可评价的生长。然而，一些抗生素受到后者培养基的影响。特别是，两种方法之间的基本协议的低水平，获得了7种抗生素。因此，两种方法需要不同的解释标准来区分耐药菌株和敏感菌株。
Lactobacilli play a crucial role as probiotics and as starter cultures in the production of fermented foods. Although lactobacilli are a technologically useful and beneficial group of bacteria, a few members of them have been rarely correlated with bacterial infections. Correspondingly, clinicians are interested in the antimicrobial susceptibility of lactobacilli. In addition, antimicrobial susceptibility testing (AST) is also relevant for commercially applied lactobacilli as bacterial strains harboring transferable antibiotic resistance genes should not be used in fermented and probiotic foods. Therefore, two methods were developed by different organizations, which were compared within this study. For this purpose, 22 Lactobacillus-type strains were tested for their antimicrobial susceptibility to 16 antibiotics following the procedures of the Clinical and Laboratory Standards Institute (CLSI) and the International Organization of Standardization (ISO)/International Dairy Federation (IDF). Crucial discrepancies between both procedures were detected mainly due to the different AST media. Hence, half of the strains tested did not consistently grow in the CLSI medium, whereas all showed evaluable growth in the ISO/IDF medium. However, some antibiotics were influenced by the latter medium. In particular, low levels of essential agreement between both methods were obtained with seven antibiotics. Accordingly, different interpretative criteria are needed for both procedures to distinguish resistant from susceptible strains.