Diversity of H-2/CO2-utilizing acetogenic bacteria from feces of non-methane-producing humans
Diversity of H-2/CO2-utilizing acetogenic bacteria from feces of non-methane-producing humans
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DOI：
10.1007/s002849900081
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发表时间：
1996-08-01
影响因子：
2.6
通讯作者：
Pochart, P
中科院分区：
文献类型：
--
作者：
Bernalier, A;Rochet, V;Pochart, P
这项工作的目的是研究低数量产甲烷古菌的非产甲烷个体结肠中利用H-2/CO2的产乙酸菌群。从4份粪便标本中分离到50株产氢产乙酸菌，以H2/CO2为唯一能源进行体外半连续培养，从中筛选出20株用于进一步研究的菌株。所有分离株均为革兰氏阳性严格厌氧菌。鉴定了不同的形态类型，为属的多样性提供了证据。所有产乙酸菌均以H-2/CO2生成醋酸盐为唯一代谢产物，符合还原产乙酸菌的化学计量方程。这些细菌还能够利用各种有机化合物进行生长。除主要产生乳酸的球菌外，葡萄糖发酵的主要最终产物为醋酸盐。酵母膏不是必需的，但对H-2/CO2的生长和产乙酸量有促进作用。
The purpose of this work was to study H-2/CO2-utilizing acetogenic population in the colons of non-methane-producing individuals harboring low numbers of methanogenic archaea. Among the 50 H-2-consuming acetogenic strains isolated from four fecal samples and an in vitro semi-continuous culture enrichment, with H-2/CO2 as sole energy source, 20 were chosen for further studies. All isolates were Gram-positive strict anaerobes. Different morphological types were identified, providing evidence of generic diversity. All acetogenic strains characterized used H-2/CO2 to form acetate as the sole metabolite, following the stoichiometric equation of reductive acetogenesis. These bacteria were also able to use a variety of organic compounds for growth. The major end product of glucose fermentation was acetate, except for strains of cocci that mainly produced lactate. Yeast extract was not necessary, but was stimulatory for growth and acetogenesis from H-2/CO2.