SKIN-DERIVED PRECURSOR SCHWANN CELL MYELINATION CAPACITY IN FOCAL TIBIAL DEMYELINATION
SKIN-DERIVED PRECURSOR SCHWANN CELL MYELINATION CAPACITY IN FOCAL TIBIAL DEMYELINATION
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DOI：
10.1002/mus.24136
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发表时间：
2014-08-01
期刊：
影响因子：
3.4
通讯作者：
Midha, Rajiv
中科院分区：
文献类型：
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作者：
Grochmal, Joey;Dhaliwal, Sundeep;Midha, Rajiv
简介：皮肤源性前体细胞（SKP）是神经嵴祖细胞，可以通过体外技术获得雪旺细胞样表型（SKP-SC）。我们假设 SKP-SC 可以产生成熟的髓磷脂，从而促进局灶性脱髓鞘损伤的恢复。方法：在双侧阿霉素损伤（0.38 μg）后 9 天，我们将 DiI 标记、产生绿色荧光蛋白 (GFP) 的 SKP-SC 单侧注射到 10 只成年 Lewis 大鼠的胫神经中（使用对侧介质对照）。胫骨复合运动动作电位 (CMAP) 随访 57 天。一个单独的形态测定队列还包括雪旺细胞注射组。结果：注射 SKP 的神经在电生理学和形态测量方面恢复最快。 SKP-SC 形成形态成熟的髓磷脂，占 SKP-SC 注射神经中总髓磷脂的 15.3 +/- 5.3%。结论：SKP-SC 具有很强的髓鞘形成能力。他们通过对一定比例的轴突进行髓鞘化来改善局灶性胫神经脱髓鞘模型的恢复。
Introduction: Skin-derived precursor cells (SKPs) are neural crest progenitor cells that can attain a Schwann cell-like phenotype through in vitro techniques (SKP-SCs). We hypothesized that SKP-SCs could produce mature myelin and, in doing so, facilitate the recovery of a focal demyelination injury. Methods: We unilaterally injected DiI-labeled, green fluorescent protein (GFP)-producing SKP-SCs into the tibial nerves of 10 adult Lewis rats (with contralateral media control), 9 days after bilateral doxorubicin injury (0.38 mu g). Tibial compound motor action potentials (CMAPs) were followed for 57 days. A separate morphometric cohort also included a Schwann cell injection group. Results: SKP-injected nerves recovered fastest in terms of electrophysiology and morphometry. SKP-SCs formed morphologically mature myelin, accounting for 15.3 +/- 5.3% of the total myelin in SKP-SC-injected nerves. Conclusions: SKP-SCs are robustly capable of myelination. They improve the recovery of a focal tibial nerve demyelination model by myelinating a measured percentage of axons.