Adhesive growth of pancreatic islet cells on a polyglycolic acid fibrous scaffold
Adhesive growth of pancreatic islet cells on a polyglycolic acid fibrous scaffold
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胰岛细胞在聚乙醇酸纤维支架上的粘附生长
DOI：
10.1016/j.transproceed.2008.02.088
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发表时间：
2008-06-01
影响因子：
0.9
通讯作者：
Song, C. F.
中科院分区：
文献类型：
--
作者：
Chun, S.;Huang, Y.;Song, C. F.
背景。利用培养的胰岛细胞治疗糖尿病具有若干优势。从理论上讲，培养的细胞具有更高的纯度和更低的免疫原性。然而，培养的胰岛细胞在体外存活率较低。在本研究中，我们将胰岛细胞种植在聚乙醇酸（PGA）纤维支架上，以促进细胞在长期培养过程中的黏附、生长和活力。
方法。从Wistar大鼠胰腺中分离出胰岛，用胶原酶消化，并通过菲可（Ficoll）法纯化。细胞在有或无PGA支架的情况下进行培养。使用双硫腙染色测定胰岛细胞纯度；使用吖啶橙 - 碘化丙啶（AO - PI）染色测定细胞活力和存活率。采用放射免疫检测法检测胰岛素分泌指数，并使用倒置显微镜和扫描电子显微镜（SEM）分析黏附支架上的细胞生长情况。
结果。与无支架对照组相比，在PGA支架上培养的细胞形态更佳，细胞死亡更少，存活时间更长。与对照细胞相比，支架上培养的细胞活力和存活率显著提高（P <.05）。在低葡萄糖（5.6 mmol/L）与高葡萄糖（16.7 mmol/L）刺激后，观察到支架上培养的细胞在培养液中的胰岛素分泌增加。两组的胰岛素分泌指数差异显著（P <.05）。扫描电子显微镜下观察到胰岛细胞生长紧密卷曲、黏附且呈三维结构。
结论。本研究结果表明，胰岛细胞能够在PGA支架上成功培养并存活。这些细胞表现出增强的活力、存活率和胰岛素分泌能力。
Background. The use of cultured pancreatic islet cells for diabetes treatment offers several advantages. In theory, cultured cells show greater purity and lower immunogenicity. However, cultured islet cells display a low survival rate in vitro. In the present study we grew islet cells on a polyglycolic acid (PGA) fibrous scaffold to promote cell adhesion, growth, and viability during prolonged culture.Methods. Islets isolated from Wistar rat pancreata were digested with collagenase and purified by the Ficoll method. Cells were grown in culture with or without PGA scaffolds. Islet cell purity was determined using a dithizone stain; viability and survival rates were determined using an AO-PI stain. The insulin-secretion index was detected using radioimmunodetection and the growth on an adhesive scaffold analyzed using an inverted microscope and scanning electron microscope (SEM).Results. In contrast to the scaffold-free control group, cells cultured on PGA scaffolds exhibited improved morphology, less cell death, and prolonged survival times. Cell viability and survival rates were significantly increased in scaffolded cells when compared to control cells (P < .05). Increased insulin secretion was observed in the culture solution of scaffolded cells following stimulation with low glucose (5.6 mmol/L) versus high glucose (16.7 mmol/L). ne secretion indices of the two groups were significantly different (P < .05). Islet cell growth, as observed under SEM, was tightly circumvolute, adhesive, and three-dimensional.Conclusions. The present results demonstrated that islet cells can successfully grow and survive in culture on a PGA scaffold. These cells exhibited enhanced viability, survival, and insulin secretion.