CONTACT MATING PHEROMONE IN BITING MIDGE, CULICOIDES-MELLEUS
CONTACT MATING PHEROMONE IN BITING MIDGE, CULICOIDES-MELLEUS
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DOI：
10.1016/0022-1910(78)90084-7
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发表时间：
1978-01-01
影响因子：
2.2
通讯作者：
CARLSON, DA
中科院分区：
文献类型：
--
作者：
LINLEY, JR;CARLSON, DA
通过长时间（5天）洗涤新鲜杀死的完整的雄蛾和雌蛾，提取了一种己烷可溶性的接触交配刺激信息素。melleus。通过允许雄性操纵（用脚）非常小的软木立方体进行试验，软木立方体上的提取物通过蒸发沉积。粗提物的硅胶色谱法得到总烃级分，当在10 ℃下测定时，女性(or. male..男性。）在同等条件下，75%或更多的男性做出了回应。稀释后的总烃馏分从女性引起显着的男性反应在1.female。合成正构烷烃的测定显示无活性，但在几种甲基取代的烷烃（2-甲基-二十二烷、8-甲基-二十二烷、9-甲基-二十三烷、10-甲基-二十二烷）中，前3种在1 μ g/模型下测定产生显著响应。
A hexane-soluble contact mating stimulant pheromone, bioassayable by sexual response to tethered males, was extracted by prolonged (5 day) washing of whole, freshly-killed male and female C. melleus. Assays were performed by allowing males to manipulate (with their feet) very small cubes of cork on which extract was deposited by evaporation. Silica-gel chromatography of crude extract yielded total hydrocarbon fractions to which, when assayed at 10 .female..female. (or .male..male.) equivalents, 75% or more of the males responded. The diluted total hydrocarbon fraction from females elicited significant male response at 1 .female. equivalent, but not at 1/10 equivalent or less. Assays of synthetic n-alkanes showed no activity, but among several methyl-substituted alkanes (2-methyl-docosane, 8-methyl-docosane, 9-methyl-tricosane, 10-methyl-docosane), the first 3 produced significant response assayed at 1 .mu.g/model.