Biosynthesis of Two Flavones, Apigenin and Genkwanin, in Escherichia coli
Biosynthesis of Two Flavones, Apigenin and Genkwanin, in Escherichia coli
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DOI：
10.4014/jmb.1503.03011
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发表时间：
2015-09-01
影响因子：
2.8
通讯作者：
Ahn, Joong-Hoon
中科院分区：
文献类型：
--
作者：
Lee, Hyejin;Kim, Bong Gyu;Ahn, Joong-Hoon
类黄酮芹菜素及其o -甲基衍生物根黄酮素具有多种生物活性，可从一些蔬菜和水果中提取。微生物是合成类黄酮的另一种选择。本研究首先利用大肠杆菌中4个基因（4CL、CHS、CHI和FNS）从对香豆酸合成芹菜素，以酪氨酸为原料合成芹菜素。通过优化不同结构的组合，芹菜素的产率由13 mg/l提高到30 mg/l。通过将两个额外的基因（TAL和POMT7）引入到产生芹菜素的大肠杆菌菌株中，我们能够从酪氨酸合成7- o -甲基芹菜素（genkwanin）。此外，过表达aroG和tyrA可调节大肠杆菌中酪氨酸的含量。工程大肠杆菌菌株合成了约41 mg/l的根管万素。
The flavonoid apigenin and its O-methyl derivative, genkwanin, have various biological activities and can be sourced from some vegetables and fruits. Microorganisms are an alternative for the synthesis of flavonoids. Here, to synthesize genkwanin from tyrosine, we first synthesized apigenin from p-coumaric acid using four genes (4CL, CHS, CHI, and FNS) in Escherichia coli. After optimization of different combinations of constructs, the yield of apigenin was increased from 13 mg/l to 30 mg/l. By introducing two additional genes (TAL and POMT7) into an apigenin-producing E. coli strain, we were able to synthesize 7-O-methyl apigenin (genkwanin) from tyrosine. In addition, the tyrosine content in E. coli was modulated by overexpressing aroG and tyrA. The engineered E. coli strain synthesized approximately 41 mg/l genkwanin.