High temperature triggers the metabolism of S-nitrosothiols in sunflower mediating a process of nitrosative stress which provokes the inhibition of ferredoxin-NADP reductase by tyrosine nitration
High temperature triggers the metabolism of S-nitrosothiols in sunflower mediating a process of nitrosative stress which provokes the inhibition of ferredoxin-NADP reductase by tyrosine nitration
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DOI：
10.1111/j.1365-3040.2011.02376.x
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发表时间：
2011-11-01
影响因子：
7.3
通讯作者：
Barroso, Juan B.
中科院分区：
文献类型：
--
作者：
Chaki, Mounira;Valderrama, Raquel;Barroso, Juan B.
高温被认为是一种主要的非生物胁迫，对植物的营养生长和生殖生长都有负面影响。然而，活性氧（ROS）的代谢是建立在HT下，很少有人知道的活性氮（RNS）的代谢。向日葵（Helianthus annuus L.）HT处理后，幼苗体内NO含量和S-亚硝基谷胱甘肽还原酶（GSNOR）活性及表达均下降，同时S-亚硝基硫醇（SNOs）和S-亚硝基谷胱甘肽（GSNO）含量增加。然而，通过高效液相色谱串联质谱（LC-MS/ MS）和共聚焦激光扫描显微镜研究的酪氨酸硝化（NO2-Tyr）的含量被诱导。高温下的硝基蛋白质组分析表明，这种应激诱导了13种酪氨酸硝化蛋白的蛋白质表达。在诱导的蛋白质中，铁氧还蛋白-NADP还原酶（FNR）被选择来评估硝化作用对其活性的影响，热应激后和体外条件下使用3-吗啉代酮亚胺（SIN-1）（过氧亚硝酸盐供体）作为硝化剂，FNR活性被抑制。总之，这些结果表明，HT增强SNO，这似乎介导蛋白质酪氨酸硝化，抑制FNR，这是参与光合作用过程。
High temperature (HT) is considered a major abiotic stress that negatively affects both vegetative and reproductive growth. Whereas the metabolism of reactive oxygen species (ROS) is well established under HT, less is known about the metabolism of reactive nitrogen species (RNS). In sunflower (Helianthus annuus L.) seedlings exposed to HT, NO content as well as S-nitrosoglutathione reductase (GSNOR) activity and expression were down-regulated with the simultaneous accumulation of total S-nitrosothiols (SNOs) including S-nitrosoglutathione (GSNO). However, the content of tyrosine nitration (NO2-Tyr) studied by high-performance liquid chromatography with tandem mass spectrometry (LC-MS/ MS) and by confocal laser scanning microscope was induced. Nitroproteome analysis under HT showed that this stress induced the protein expression of 13 tyrosine-nitrated proteins. Among the induced proteins, ferredoxin-NADP reductase (FNR) was selected to evaluate the effect of nitration on its activity after heat stress and in vitro conditions using 3-morpholinosydnonimine (SIN-1) (peroxynitrite donor) as the nitrating agent, the FNR activity being inhibited. Taken together, these results suggest that HT augments SNOs, which appear to mediate protein tyrosine nitration, inhibiting FNR, which is involved in the photosynthesis process.