Polyketides from the Mangrove-Derived Endophytic Fungus Nectria sp. HN001 and Their α-Glucosidase Inhibitory Activity.
Polyketides from the Mangrove-Derived Endophytic Fungus Nectria sp. HN001 and Their α-Glucosidase Inhibitory Activity.
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红树林内生真菌 Nectria sp HN001 的聚酮化合物及其 α-葡萄糖苷酶抑制活性
DOI：
10.3390/md14050086
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发表时间：
2016-04-28
期刊：
影响因子：
5.4
通讯作者：
She Z
中科院分区：
文献类型：
--
作者：
Cui H;Liu Y;Nie Y;Liu Z;Chen S;Zhang Z;Lu Y;He L;Huang X;She Z
从内生真菌Nectria sp.的培养物中分离得到4个新的多酮化合物：蜜环酸A-C(1-3)和12-表位异香豆素(4)，以及3个已知化合物：柠檬酸异香豆素(5)、柠檬酸异香豆素(6)和大果糖C(7)。从采自南中国海的红树林植物卵形海桑(Sonneratia Ovata)的一个新鲜枝条中分离得到HN001。通过对核磁共振和质谱学数据的详细分析，确定了它们的结构。用Mosher酯法对化合物4的立体碳的绝对构型进行了进一步的指认。所有化合物均具有α-葡萄糖苷酶抑制活性，其中新化合物2和3具有较强的抑制活性，其IC50值分别为23.5和42.3μM，均高于阳性对照(阿卡波糖、IC50值分别为815.3μM)。
Four new polyketides: nectriacids A–C (1–3) and 12-epicitreoisocoumarinol (4), together with three known compounds: citreoisocoumarinol (5), citreoisocoumarin (6), and macrocarpon C (7) were isolated from the culture of the endophytic fungus Nectria sp. HN001, which was isolated from a fresh branch of the mangrove plant Sonneratia ovata collected from the South China Sea. Their structures were determined by the detailed analysis of NMR and mass spectroscopic data. The absolute configuration of the stereogenic carbons for compound 4 was further assigned by Mosher’s ester method. All of the isolated compounds were tested for their α-glucosidase inhibitory activity by UV absorbance at 405 nm, and new compounds 2 and 3 exhibited potent inhibitory activity with IC50 values of 23.5 and 42.3 μM, respectively, which were more potent than positive control (acarbose, IC50, 815.3 μM).