Anaerostipes hadrus comb. nov., a dominant species within the human colonic microbiota; reclassification of Eubacterium hadrum Moore et al. 1976
Anaerostipes hadrus comb. nov., a dominant species within the human colonic microbiota; reclassification of Eubacterium hadrum Moore et al. 1976
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DOI：
10.1016/j.anaerobe.2012.09.002
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发表时间：
2012-10-01
期刊：
影响因子：
2.3
通讯作者：
Lawson, Paul A.
中科院分区：
文献类型：
--
作者：
Allen-Vercoe, Emma;Daigneault, Michelle;Lawson, Paul A.
最近的分子分析表明，与Ss2/1和Ssc/2菌株相关的细菌代表了在成人粪便样本中发现的10个最丰富的系统型之一。这两株细菌是从一名健康人的粪便样本中分离出来的利用D-乳酸的细菌。我们在这里表明，它们在16SRRNA基因序列上与从克罗恩病患者的结肠活检中发现的一株新的丁酸产生菌有99.9%的相似性，也与最近报道的霍德姆真核细菌ATCC 29173(T)的序列相似。使用API Rapid ID 32A和API Zym测试系统进行的生化图谱分析证实了与E.hadrum ATCC 29173(T)非常接近的表型相似性。但也指出，对该物种的描述应扩大到包括从D-乳酸和乙酸盐生产丁酸盐的能力。系统发育分析证实，该菌与无氧菌属的成员有亲缘关系(92.3-94.2%的序列相似性)，属于乳螺科(原为梭状芽孢杆菌簇XlVa)。根据系统发育、表型和化学分类的证据，建议将该种划入AnAerostipe属，命名为AnAerostipe hadrus comb。11月该菌株是一种典型的梭状曲霉。11月IS=ATCC 29173(T)(=DSM3319(T)=VP 82-52(T))。(C)2012爱思唯尔有限公司。保留所有权利。
Recent molecular analyses suggest that bacteria related to strains SS2/1 and SSC/2, previously reported to be distantly related to Anaerostipes caccae NCIMB 13811(T), represent one of the ten most abundant phylotypes detected in adult human faecal samples. These two strains were isolated as D-lactate-utilizing bacteria from faecal samples of a healthy individual. We show here that they share >99.9% similarity in 16S rRNA gene sequence with a new butyrate-producing isolate recovered from a colonic biopsy of a Crohn's disease patient, and also with the sequence reported recently for Eubacterium hadrum ATCC 29173(T). Biochemical profiling using API Rapid ID 32A and API ZYM test systems confirmed a close phenotypic similarity to E. hadrum ATCC 29173(T). but also indicated that the description of this species should be expanded to include the ability to produce butyrate from D-lactate and acetate. Phylogenetic analysis confirmed an affinity between E. hadrum and members of the genus Anaerostipes (92.3-94.2% sequence similarity) belonging to the family Lachnospiraceae (formerly Clostridium cluster XlVa). Based on phylogenetic, phenotypic and chemotaxonomic evidence it is proposed that E. hadrum be transferred to the genus Anaerostipes with the name Anaerostipes hadrus comb. nov. The type strain of A. hadrus comb. nov. is =ATCC 29173(T) (=DSM 3319(T) = VP 82-52(T)). (C) 2012 Elsevier Ltd. All rights reserved.