Identification of Bacterial Small RNAs by RNA Sequencing
Identification of Bacterial Small RNAs by RNA Sequencing
复制标题
DOI：
10.1007/978-1-4939-0473-0_34
复制
发表时间：
2014-01-01
期刊：
影响因子：
--
通讯作者：
Long, Katherine S.
中科院分区：
文献类型：
--
作者：
Gomez-Lozano, Maria;Marvig, Rasmus Lykke;Long, Katherine S.
已知细菌中的小调节RNA（sRNA）调节基因表达并控制多种过程，包括代谢反应、应激反应和响应于环境信号的发病机制。描述了一种基于RNA测序（RNA-seq）在全基因组范围内鉴定细菌sRNA的方法，该方法涉及三种不同测序文库的制备和分析。由于在每个文库中鉴定了显著数量的独特sRNA，因此可以单独或组合使用文库以增加鉴定的sRNA的数量。该方法可以应用于在不同生长和胁迫条件下鉴定任何细菌中的sRNA。
Small regulatory RNAs (sRNAs) in bacteria are known to modulate gene expression and control a variety of processes including metabolic reactions, stress responses, and pathogenesis in response to environmental signals. A method to identify bacterial sRNAs on a genome-wide scale based on RNA sequencing (RNA-seq) is described that involves the preparation and analysis of three different sequencing libraries. As a significant number of unique sRNAs are identified in each library, the libraries can be used either alone or in combination to increase the number of sRNAs identified. The approach may be applied to identify sRNAs in any bacterium under different growth and stress conditions.