Formation of water-soluble metal cyanide complexes from solid minerals by Pseudomonas plecoglossicida
Formation of water-soluble metal cyanide complexes from solid minerals by Pseudomonas plecoglossicida
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DOI：
10.1111/j.1574-6968.2006.00245.x
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发表时间：
2006-06-01
影响因子：
2.1
通讯作者：
Brandl, H
中科院分区：
文献类型：
--
作者：
Faramarzi, MA;Brandl, H
一些假单胞菌能够形成氢氰酸（HCN），特别是在富含甘氨酸的条件下生长时。在有金属存在的情况下，氰化物能形成化学稳定性高的水溶性金属配合物。我们研究了利用形成hcn的微生物从固体矿物中动员金属作为氰化物配合物的可能性。在含铜和含镍固体矿物的环境中培养了假单胞菌。在粉末状元素镍上，HCN在培养前12 h内快速生成，并形成水溶性的四氰镍酸盐。铜矿、橄榄石、黄铜矿、孔雀石、斑铜矿、绿松石、针石、镍黄石以及电子碎片也进行了生物处理。当P. pleclossisidida生长在铜或钛矿存在下时，氰化物络合铜的最大浓度分别对应42%和27%的增溶作用。晶体体系、金属氧化态和矿物疏水性可能对金属的动员有重要影响。然而，不可能将金属动员分配给单一矿物属性。在人工切割电路板的生长过程中检测到氰化物络合金。最大双氰酸盐浓度对应于总金的溶解度为68.5%。基于某些微生物形成HCN的能力，这些发现代表了一种新型的微生物从固体矿物中动员镍和铜。
A few Pseudomonas species are able to form hydrocyanic acid (HCN), particularly when grown under glycine-rich conditions. In the presence of metals, cyanide can form water-soluble metal complexes of high chemical stability. We studied the possibility to mobilize metals as cyanide complexes from solid minerals using HCN-forming microorganisms. Pseudomonas plecoglossicida was cultivated in the presence of copper- and nickel-containing solid minerals. On powdered elemental nickel, fast HCN generation within the first 12 h of incubation was observed and water-soluble tetracyanaonickelate was formed. Cuprite, tenorite, chrysocolla, malachite, bornite, turquoise, millerite, pentlandite as well as shredded electronic scrap was also subjected to a biological treatment. Maximum concentrations of cyanide-complexed copper corresponded to a solubilization of 42% and 27% when P. plecoglossicida was grown in the presence of cuprite or tenorite, respectively. Crystal system, metal oxidation state and mineral hydrophobicity might have a significant influence on metal mobilization. However, it was not possible to allocate metal mobilization to a single mineral property. Cyanide-complexed gold was detected during growth on manually cut circuit boards. Maximum dicyanoaurate concentration corresponded to a 68.5% dissolution of the total gold added. These findings represent a novel type of microbial mobilization of nickel and copper from solid minerals based on the ability of certain microbes to form HCN.