Coomassie brilliant dyes as surface-enhanced Raman scattering probes for protein-ligand recognitions.
Coomassie brilliant dyes as surface-enhanced Raman scattering probes for protein-ligand recognitions.
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DOI：
10.1021/ac100202w
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发表时间：
2010-04
影响因子：
7.4
通讯作者：
X. Han;Lei Chen;Jie Guo;Bing Zhao;Y. Ozaki
中科院分区：
文献类型：
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作者：
X. Han;Lei Chen;Jie Guo;Bing Zhao;Y. Ozaki
考马斯亮蓝染料具有与蛋白质的高亲和性和高拉曼活性，基于此，我们采用亮蓝R-250（BBR）和亮蓝G-250（BBG）作为表面增强拉曼散射（Sers）标记物来探测蛋白质-配体的相互作用。该方法与以前提出的方法的不同之处在于，在生物纯化之前快速标记靶蛋白，而无需分离和纯化程序，而不是将拉曼标记物附着到金属纳米颗粒上，这显著简化了拉曼染料标记程序。在典型的测定中，配体官能化的金属纳米颗粒通过靶蛋白特异性结合组装，并且该组装顺序地开启所提出的标记的拉曼散射的电磁增强。该方法具有快速、高灵敏度和光谱多路复用等优点，在蛋白质-蛋白质和蛋白质-小分子体系的研究中具有很大的潜力，不仅适用于溶液体系，而且适用于柔性固体基质。
Coomassie brilliant dyes have high affinity to proteins and high Raman activity, and on the basis of which, we have employed brilliant blue R-250 (BBR) and brilliant blue G-250 (BBG) as surface-enhanced Raman scattering (SERS) labels to probe protein-ligand recognitions. This method differs from previously proposed methods in that target proteins are labeled rapidly before biological recognitions without procedures of separation and purification, rather than attaching Raman labels to metal nanoparticles, which significantly simplifies the Raman dye labeling procedure. In typical assays, ligand-functionalized metal nanoparticles assemble by target protein-specific bindings and this assembly sequentially turns on electromagnetic enhancement of Raman scattering of the proposed labels. The method with its advantages of rapidness, high sensitivity, and spectral multiplexing has great potential in probing protein-protein and protein-small molecule recognitions not only in solution systems but also on flexible solid substrates.