Experimental periodontitis promotes transient vascular inflammation and endothelial dysfunction
Experimental periodontitis promotes transient vascular inflammation and endothelial dysfunction
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DOI：
10.1016/j.archoralbio.2013.03.009
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发表时间：
2013-09-01
影响因子：
3
通讯作者：
Fernandes, Daniel
中科院分区：
文献类型：
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作者：
Brito, Lorena C. W.;DalBo, Silvia;Fernandes, Daniel
目的：本研究旨在评估大鼠实验性牙周炎引起的全身炎症反应和心血管变化。设计：实验性牙周炎通过将棉花结扎在每只雄性大鼠的双侧下颌第一磨牙和上颌第二磨牙的颈部周围来诱导。假手术大鼠在手术后立即去除结扎线。术后7、14或28天，评价乙酰胆碱、硝普钠和苯丙氨酸对血压、主动脉环和离体和灌注肠系膜床的影响。取血进行血浆白细胞介素-6（IL-6）、C反应蛋白（CRP）和血脂评价。肠系膜血管获得评估超氧化物的产生和一氧化氮合酶3（NOS-3）expression.Results：结扎引起的牙周炎减少内皮依赖性血管舒张，内皮功能障碍的标志。这种作用与全身炎症标志物（IL-6和CRP）的增加、脂质谱的改变、血管超氧化物产生的增加和NOS-3表达的减少有关。值得注意的是，这些影响中有许多是暂时的。结论：牙周炎引起短暂的全身和血管炎症，导致内皮功能障碍，心血管疾病的第一步。此外，牙周炎的动物模型，这里使用的可能是一个有价值的工具，研究牙周炎和内皮功能障碍之间的关系。(C)2013爱思唯尔有限公司保留所有权利。
Objectives: This study aimed to evaluate the systemic inflammatory response and cardiovascular changes induced by experimental periodontitis in rats.Design: Experimental periodontitis was induced by placing a cotton ligature around the cervix of both sides of mandibular first molars and maxillary second molars in each male rat. Sham-operated rats had the ligature removed immediately after the procedure. Seven, 14 or 28 days after procedure, the effects of acetylcholine, sodium nitroprusside and phenylephrine were evaluated on blood pressure, aortic rings and isolated and perfused mesenteric bed. The blood was obtained for plasma Interleukin-6 (IL-6), C-reactive protein (CRP) and lipid evaluation. The mesenteric vessels were obtained to evaluate superoxide production and nitric oxide synthase 3 (NOS-3) expression.Results: Ligature induced periodontitis reduced endothelium-dependent vasodilatation, a hallmark of endothelial dysfunction. This effect was associated with an increase in systemic inflammatory markers (IL-6 and CRP), worsens on lipid profile, increased vascular superoxide production and reduced NOS-3 expression. It is interesting to note that many of these effects were transitory.Conclusion: Periodontitis induced a transient systemic and vascular inflammation which leads to endothelial dysfunction, an initial step for cardiovascular diseases. Moreover, the animal model of periodontitis used here may represent a valuable tool for studying the relationship between periodontitis and endothelial dysfunction. (C) 2013 Elsevier Ltd. All rights reserved.