Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs.
Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs.
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DOI：
10.1186/1471-213x-10-94
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发表时间：
2010-09-02
影响因子：
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通讯作者：
Corominas M
中科院分区：
文献类型：
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作者：
Blanco E;Ruiz-Romero M;Beltran S;Bosch M;Punset A;Serras F;Corominas M
再生是生物体重建受损或截肢的身体部位的能力，可以使用模式生物在分子水平上进行研究。果蝇成虫盘是成虫表皮结构的幼虫原基，在移植和体内培养到成虫腹部后能够进行再生生长。使用表达谱分析，我们研究了再生行为的翅盘在0，24和72小时后，碎片和植入成年女性。基于表达水平，我们生成了一个在翅盘再生中具有假定作用的基因目录，将其鉴定为四类：1）在前24小时内差异表达的基因; 2）在24和72小时之间差异表达的基因; 3）在两个时间段之间表达水平显著变化的基因; 4）在整个再生过程中其表达水平持续增加或减少的基因。在这些基因中，我们确定了JNK和Notch信号通路和染色质调节因子的成员。通过计算分析，我们认识到这些途径的下游转录因子的假定结合位点在多个果蝇中是保守的，表明不同基因类的成员之间的潜在关系。遗传突变体的实验数据提供了证据，在翅盘再生的选择基因的要求。我们已经能够区分参与再生过程早期和晚期步骤的各种类型的基因。我们的数据表明，整合的信号转导途径的启动子调控基因。
Regeneration is the ability of an organism to rebuild a body part that has been damaged or amputated, and can be studied at the molecular level using model organisms. Drosophila imaginal discs, which are the larval primordia of adult cuticular structures, are capable of undergoing regenerative growth after transplantation and in vivo culture into the adult abdomen. Using expression profile analyses, we studied the regenerative behaviour of wing discs at 0, 24 and 72 hours after fragmentation and implantation into adult females. Based on expression level, we generated a catalogue of genes with putative role in wing disc regeneration, identifying four classes: 1) genes with differential expression within the first 24 hours; 2) genes with differential expression between 24 and 72 hours; 3) genes that changed significantly in expression levels between the two time periods; 4) genes with a sustained increase or decrease in their expression levels throughout regeneration. Among these genes, we identified members of the JNK and Notch signalling pathways and chromatin regulators. Through computational analysis, we recognized putative binding sites for transcription factors downstream of these pathways that are conserved in multiple Drosophilids, indicating a potential relationship between members of the different gene classes. Experimental data from genetic mutants provide evidence of a requirement of selected genes in wing disc regeneration. We have been able to distinguish various classes of genes involved in early and late steps of the regeneration process. Our data suggests the integration of signalling pathways in the promoters of regulated genes.