flk2/flt3 ligand is a potent cofactor for the growth of primitive B cell progenitors.
flk2/flt3 ligand is a potent cofactor for the growth of primitive B cell progenitors.
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DOI：
10.4049/jimmunol.156.2.489
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发表时间：
1996-01
影响因子：
4.4
通讯作者：
B. Hunte;S. Hudak;D. Campbell;Yuming Xu;Donna M. Rennick
中科院分区：
文献类型：
--
作者：
B. Hunte;S. Hudak;D. Campbell;Yuming Xu;Donna M. Rennick
我们研究了flk 2/flt 3配体（FL）在B细胞淋巴细胞生成中的作用。FL刺激未成熟B细胞生长的能力使用不同的群体进行评估：CD 43低B220+前B细胞、CD 43 +B220+前B细胞和CD 43 + B220低祖细胞。FL不能影响pro-B或pre-B细胞的生长，无论是单独使用还是与干细胞因子（SCF）或IL-7组合使用。与此形成鲜明对比的是，FL是CD 43 + B220 low祖细胞的有效辅因子，与IL-7和/或SCF相互作用以刺激其生长。FL与IL-7加SCF的组合刺激了这些细胞的最大扩增，尽管小于基质细胞培养物中的刺激。当基于热稳定Ag（CD 24）的表达将CD 43 + B220低祖细胞分为CD 24-和CD 24+亚群时，FL应答细胞仅包含在CD 24-亚群内。FL与SCF或IL-7相互作用以刺激它们的生长，分别导致细胞结构增加20倍和50倍。由于CD 24亚群是研究的B细胞群中最不成熟的，我们的数据表明FL共刺激非常原始的B祖细胞的扩增。
We have investigated the role of flk2/flt3 ligand (FL) in B cell lymphopoiesis. The ability of FL to stimulate the growth of immature B cells was assessed using distinct populations: CD43lowB220+ pre-B cells, CD43+B220+ pro-B cells, and CD43+B220low progenitors. FL failed to affect the growth of the pro-B or pre-B cells whether used alone or in combination with stem cell factor (SCF) or IL-7. In striking contrast, FL was a potent cofactor for the CD43+B220low progenitor cells, interacting with either IL-7 and/or SCF to stimulate their growth. The combination of FL with IL-7 plus SCF stimulated maximum expansion of these cells, albeit, less than that stimulated in stromal cell cultures. When the CD43+B220low progenitors were divided based on expression of heat stable Ag (CD24) into a CD24- and a CD24+ subset, the FL-responsive cells were contained only within the CD24- subset. FL interacted with SCF or with IL-7 to stimulate their growth resulting in a 20- and 50-fold increase in cellularity, respectively. Since the CD24- subset was the most immature of the B cell populations studied, our data suggest that FL costimulates the expansion of very primitive B cell progenitors.