Solubilization and stable dispersion of myofibrillar proteins in water through the destruction and inhibition of the assembly of filaments using high-intensity ultrasound
Solubilization and stable dispersion of myofibrillar proteins in water through the destruction and inhibition of the assembly of filaments using high-intensity ultrasound
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DOI：
10.1016/j.ultsonch.2020.105160
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发表时间：
2020-10-01
影响因子：
8.4
通讯作者：
Kong, Baohua
中科院分区：
文献类型：
--
作者：
Liu, Haotian;Zhang, Huan;Kong, Baohua
肌原纤维蛋白在水中的不溶性和分散性差一直是限制新型肉制品开发的主要因素。本研究旨在探讨不同功率（0、150、300、450和600 W）的高强度超声（HIU）改善MP在水中溶解度和分散稳定性的机制。溶解度分析表明，HIU显著增加了MPs的水溶性（p < 0.05）。经450 W处理的MP在水中的分散稳定性最好，zeta电位最高，粒径最小，分布最均匀（p < 0.05）。基于圆二色谱和荧光光谱和表面疏水性分析，MP超螺旋的损失和随后的丝状肌球蛋白结构的随机解离似乎是MP增溶的主要机制。此外，根据ζ-电位、SDS-PAGE和Nano LC-ESI-MS/MS分析，表面电荷的增加和可溶性低聚物的形成可以提供额外的力来抑制细丝组装，从而改善水性MP悬浮液的稳定性。原子力显微镜（AFM）的观察进一步证实了这些结果。总之，HIU处理有效地改善了MP在水中的溶解度和分散稳定性。
The insolubility and poor dispersion of myofibrillar proteins (MPs) in water have always been the primary factors limiting the development of novel meat-based products. This study aimed to explore the mechanisms by which high-intensity ultrasound (HIU) at various power settings (0, 150, 300, 450 and 600 W) improved the solubility and dispersion stability of MPs in water. According to the solubility analysis, HIU significantly increased the water solubility of MPs (p < 0.05). The MPs treated with 450 W exhibited the best dispersion stability in water, which corresponded to the highest zeta-potential, smallest particle size and most uniform distribution (p < 0.05). Based on the circular dichroism and fluorescence spectroscopy and surface hydro-phobicity analysis, the loss of the MP superhelix and subsequent random dissociation of the filamentous myosin structure appeared to be the main mechanism of MP solubilization. In addition, according to the zeta-potential, SDS-PAGE and Nano LC-ESI-MS/MS analyses, the increase in surface charge and the formation of soluble oligomers may provide additional forces to inhibit filament assembly, thereby improving the stability of the aqueous MP suspension. Atomic force microscopy (AFM) observations further confirmed these results. In conclusion, an HIU treatment effectively improves the solubility and dispersion stability of MP in water.