CLONING OF THE CANDIDA-GLABRATA TRP1 AND HIS3 GENES, AND CONSTRUCTION OF THEIR DISRUPTANT STRAINS BY SEQUENTIAL INTEGRATIVE TRANSFORMATION
CLONING OF THE CANDIDA-GLABRATA TRP1 AND HIS3 GENES, AND CONSTRUCTION OF THEIR DISRUPTANT STRAINS BY SEQUENTIAL INTEGRATIVE TRANSFORMATION
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DOI：
10.1016/0378-1119(95)00552-h
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发表时间：
1995-11-20
期刊：
影响因子：
3.5
通讯作者：
ARISAWA, M
中科院分区：
文献类型：
--
作者：
KITADA, K;YAMAGUCHI, E;ARISAWA, M
光滑念珠菌（Cg）TRP 1和HIS 3基因已分别通过互补酿酒酵母（Sc）trp 1和his 3突变体分离。Cg TRP 1编码217个氨基酸的多肽，其氨基酸序列与Sc TRP 1的氨基酸序列具有58%的同源性。Cg HIS 3编码210个氨基酸的多肽，其氨基酸序列与Sc HIS 3的氨基酸序列具有73%的同源性。通过连续整合转化破坏Cg TRP 1和HIS 3，其中Sc URA 3用作转化的选择标记。使用所得到的his 3（-）和trp 1（-）营养缺陷型菌株来检查Sc基因补充Cg突变的能力; Sc HIS 3和TRP 1分别补充Cg his 3（-）和trp 1（-）突变。
The Candida glabrata (Cg) TRP1 and HIS3 genes have been isolated by complementation of the Saccharomyces cerevisiae (Sc) trp1 and his3 mutants, respectively. Cg TRP1 encodes a polypeptide of 217 amino acids (aa), whose aa sequence is 58% identical to that of Sc TRP1. Cg HIS3 encodes a polypeptide of 210 aa, whose aa sequence is 73% identical to that of the Sc HIS3. Both Cg TRP1 and HIS3 were disrupted by sequential integrative transformation where the Sc URA3 was used as a selection marker for transformation. The resulting auxotrophic strain of his3(-) and trp1(-) was used to examine the ability of the Sc genes to complement the Cg mutations; Sc HIS3 and TRP1 complemented the Cg his3(-) and trp1(-) mutations, respectively.