The superoxide dismutases of Candida glabrata protect against oxidative damage and are required for lysine biosynthesis, DNA integrity and chronological life survival
The superoxide dismutases of Candida glabrata protect against oxidative damage and are required for lysine biosynthesis, DNA integrity and chronological life survival
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DOI：
10.1099/mic.0.000006
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发表时间：
2015-02-01
期刊：
影响因子：
2.8
通讯作者：
De Las Penas, Alejandro
中科院分区：
文献类型：
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作者：
Briones-Martin-del-Campo, Marcela;Orta-Zavalza, Emmanuel;De Las Penas, Alejandro
真菌病原体光滑念珠菌具有明确的氧化应激反应，对氧化应激具有极强的抵抗力，并且可以在吞噬细胞内存活。为了进一步了解C.光果苔属中的Cu、ZnSOD（Sod 1）和MnSOD（Sod 2）。我们发现，Sod 1是总SOD活性的主要贡献者，存在于细胞质中，而Sod 2是线粒体！蛋白这两种SOD在氧化应激反应中发挥了核心作用，但Sod 1在发酵生长过程中更重要，Sod 2在呼吸和非发酵碳源生长过程中更重要。有趣的是，C。glabrata细胞缺乏这两种SOD表现出赖氨酸营养缺陷型，自发突变率高，按时间顺序寿命减少。因此，我们的研究揭示了SOD在C.光滑的
The fungal pathogen Candida glabrata has a well-defined oxidative stress response, is extremely resistant to oxidative stress and can survive inside phagocytic cells. In order to further our understanding of the oxidative stress response in C. glabrata, we characterized the superoxide dismutases (SODs) Cu,ZnSOD (Sod1) and MnSOD (Sod2). We found that Sod1 is the major contributor to total SOD activity and is present in cytoplasm, whereas Sod2 is a mitochondria! protein. Both SODs played a central role in the oxidative stress response but Sod1 was more important during fermentative growth and Sod2 during respiration and growth in non-fermentable carbon sources. Interestingly, C. glabrata cells lacking both SODs showed auxotrophy for lysine, a high rate of spontaneous mutation and reduced chronological lifespan. Thus, our study reveals that SODs play an important role in metabolism, lysine biosynthesis, DNA protection and aging in C. glabrata.