Effect of fabA and desA Mediated Low Temperature Adaption on Shewanella putrefaciens via Regulation of Unsaturated Fatty Acid Metabolism
Effect of fabA and desA Mediated Low Temperature Adaption on Shewanella putrefaciens via Regulation of Unsaturated Fatty Acid Metabolism
复制标题
fabA 和 desA 通过调节不饱和脂肪酸代谢介导的低温适应对腐败希瓦氏菌的影响
DOI：
10.1166/nnl.2019.3023
复制
发表时间：
2019-10
影响因子：
--
通讯作者：
Jing Xie
中科院分区：
文献类型：
--
作者：
Li Chen;Shengping Yang;Yunfang Qian;Jing Xie
腐坏希瓦氏菌是低温冷藏水产品中的一种腐败菌，严重威胁着人类健康和水产养殖。腐臭链球菌细胞膜的脂肪酸组成已被证明参与了细菌的适应
Shewanella putrefaciensis a kind of spoilage bacteria in low temperature chilled aquatic products, which seriously threats human health and aquaculture. The fatty acid composition of S. putrefaciens cell membranes has been shown to be involved in adaption of bacteria to
various environments. However, the specific fatty acid metabolism of S. putrefaciens to the low temperature environment remains unknown. In this study, the growth of S. putrefaciens, the response of fatty acid composition to low temperature production, and the differential expression
and synthesis of enzymes related to unsaturated fatty acid synthesis were investigated by lack of fabA and desA in S. putrefaciens. Results showed that loss of fabA and desA suppressed the growth of S. putrefaciens and reduced unsaturated fatty acid contents at low temperature.
In addition, the upregulation of fabA, but not desA resulted in accumulation of unsaturated fatty acid. Up-regulations of fabA and desA both resulted in promotion of GPR41 and Retn gene and protein expressions. These results demonstrated that the deletions of fabA and desA resulted in reduction
of unsaturated fatty acid and key downstream genes of fatty acid metabolism, which suggested that unsaturated fatty acid was involved in the adaptations of fabA and desA-mediated S. putrefaciens to the low temperature environment. These results provided a tentative mechanism of the
synthesis of unsaturated fatty acids in S. putrefaciens under low temperature conditions.