A single regulatory region modulates both cis activation and trans activation of the herpes simplex virus VP5 promoter in transient-expression assays in vivo.
A single regulatory region modulates both cis activation and trans activation of the herpes simplex virus VP5 promoter in transient-expression assays in vivo.
复制标题
在体内瞬时表达测定中，单个调节区可调节单纯疱疹病毒 VP5 启动子的顺式激活和反式激活。
DOI：
10.1128/jvi.60.2.460-469.1986
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发表时间：
1986
影响因子：
5.4
通讯作者：
Wagner,EK
中科院分区：
文献类型：
--
作者：
Blair,ED;Wagner,EK
对由单纯疱疹病毒主要衣壳蛋白 (VP5) 启动子控制的细菌氯霉素乙酰转移酶基因表达的详细分析表明，该启动子可以在功能上分为 80 个碱基的核心区和大约 100 个碱基的调节区，该核心区具有充当 pol II 启动子所需的最少信息，但不会被病毒重复感染或通过带有功能性 α（立即早期）基因的质粒的共转染完全激活。核心上游，这允许完全诱导 VP5 启动子驱动的氯霉素乙酰转移酶活性，但抑制 VP5 核心启动子被猿猴病毒 40 增强子顺式激活的能力。这与碱性核酸外切酶启动子（一种早期启动子模型）的情况形成鲜明对比，并定义了该启动子的区域，可用于研究病毒启动子与病毒感染诱导的假定调节蛋白之间的相互作用。
A detailed analysis of the expression of the bacterial chloramphenicol acetyltransferase gene controlled by the herpes simplex virus major capsid protein (VP5) promoter showed that this promoter can be functionally separated into an 80-base core region, which has the minimal information required to serve as a pol II promoter but which is not fully activated by viral superinfection or by cotransfections with plasmids bearing functional alpha (immediate-early) genes, and an approximately 100-base regulatory region upstream of the core, which allowed full induction of VP5 promoter-driven chloramphenicol acetyltransferase activity but which repressed the ability of the VP5 core promoter to be cis activated by the simian virus 40 enhancer. This was in distinct contrast to the situation with the alkaline exonuclease promoter (a model early promoter) and defined the regions of this promoter which can be used to study the interaction between viral promoters and putative regulatory proteins induced by viral infection.