Effect of gestational hyperglycemia on insulin secretion in vivo and in vitro by fetal rat pancreas.
Effect of gestational hyperglycemia on insulin secretion in vivo and in vitro by fetal rat pancreas.
复制标题
妊娠期高血糖对胎鼠胰腺体内外胰岛素分泌的影响。
作者：
M. Bihoreau;A. Ktorza;A. Kervran;L. Picon
在妊娠最后一周使用持续葡萄糖输注使未受限制的妊娠大鼠产生轻度或高度高血糖的近期胎儿中研究妊娠高血糖对B细胞功能的影响。在胎儿中研究了胰腺和血浆胰岛素以及体外胰岛素分泌。与对照组相比，轻度高血糖胎儿的胰腺和血浆胰岛素浓度增加，并且在体外对葡萄糖的反应中胰岛素释放相似。在高血糖胎儿中，胰腺和血浆胰岛素浓度与对照组相比没有变化，体外胰岛素释放对葡萄糖和葡萄糖加茶碱的混合物不敏感。这些结果证实，葡萄糖能够刺激正常或轻度高血糖胎儿的胰岛素分泌，并表明严重的高血糖本身，没有其他代谢紊乱或毒性损伤的关联，深刻地改变了胎鼠B细胞的刺激-分泌偶联。
The effects of gestational hyperglycemia on B-cell function were studied in near-term fetuses from unrestrained pregnant rats made slightly or highly hyperglycemic using continuous glucose infusion during the last week of pregnancy. Pancreatic and plasma insulin and insulin secretion in vitro were studied in the fetuses. Compared with controls, slightly hyperglycemic fetuses showed increased pancreatic and plasma insulin concentrations and similar insulin release in response to glucose in vitro. In highly hyperglycemic fetuses, pancreatic and plasma insulin concentrations were unchanged compared with controls, and insulin release in vitro was insensitive to glucose and to the mixture glucose plus theophylline. These results confirm that glucose is able to stimulate insulin secretion in normal or slightly hyperglycemic fetuses and suggest that severe hyperglycemia per se, without association of other metabolic disorders or toxic injuries, profoundly alters the stimulus-secretion coupling of the fetal rat B-cell.