MOLECULAR DIVERSITY OF CA2+ CHANNEL-ALPHA-1 SUBUNITS FROM THE MARINE RAY DISCOPYGE-OMMATA
MOLECULAR DIVERSITY OF CA2+ CHANNEL-ALPHA-1 SUBUNITS FROM THE MARINE RAY DISCOPYGE-OMMATA
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DOI：
10.1073/pnas.90.9.3787
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发表时间：
1993-05-01
影响因子：
11.1
通讯作者：
SCHWARZ, TL
中科院分区：
文献类型：
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作者：
HORNE, WA;ELLINOR, PT;SCHWARZ, TL
在许多神经元中，突触前末梢的递质释放是通过二氢吡啶不敏感的Ca 2+通道的Ca 2+内流触发的。我们已经在海洋射线Discopyge ommata的神经系统中寻找这种通道的cDNA。一个cDNA（doe-2）类似于二氢吡啶敏感的L型通道，并且两个cDNA（doe-1和doe-4）更类似于二氢吡啶不敏感的非L型通道的亚家族。doe-4编码2326个氨基酸的蛋白质，最接近于先前克隆的N型通道。doe-1编码2223个氨基酸的蛋白质，是非L型通道的独立分支的成员。北方印迹分析表明，doe-1是丰富的前脑。DOE-4在电叶中更丰富，因此可以控制运动神经末梢中的神经递质释放。这些结果表明，在4亿年前高等和低等脊椎动物分化之前的进化阶段，Ca 2+通道基因的家族模式已经被保留下来。这些通道的克隆可能是一个有用的起点，阐明的作用，在神经末梢的兴奋分泌耦合的Ca 2+通道。
In many neurons, transmitter release from presynaptic terminals is triggered by Ca2+ entry via dihydropyridine-insensitive Ca2+ channels. We have looked for cDNAs for such channels in the nervous system of the marine ray Discopyge ommata. One cDNA (doe-2) is similar to dihydropyridine-sensitive L-type channels, and two cDNAs (doe-1 and doe-4) are more similar to the subfamily of dihydropyridine-insensitive non-L-type channels. doe-4, which encodes a protein of 2326 aa, most closely resembles a previously cloned N-type channel. doe-1, which encodes a protein of 2223 aa, is a member of a separate branch of the non-L-type channels. Northern blot analysis reveals that doe-1 is abundant in the forebrain. doe-4 is more plentiful in the electric lobe and, therefore, may control neurotransmitter release in motor nerve terminals. These results show that the familial pattern of Ca2+-channel genes has been preserved from a stage in evolution before the divergence of higher and lower vertebrates >400 million years ago. The cloning of these channels may be a useful starting point for elucidating the role of the Ca2+ channels in excitation-secretion coupling in nerve terminals.