Erythrocyte membrane-coated gold nanocages for targeted photothermal and chemical cancer therapy
Erythrocyte membrane-coated gold nanocages for targeted photothermal and chemical cancer therapy
复制标题
用于靶向光热和化学癌症治疗的红细胞膜涂层金纳米笼。
DOI：
10.1088/1361-6528/aa9ca1
复制
发表时间：
2018-02-23
期刊：
影响因子：
3.5
通讯作者：
Liu, Wei
中科院分区：
文献类型：
--
作者：
Zhu, Dao-Ming;Xie, Wei;Liu, Wei
近年来，红细胞膜包裹的纳米粒子因其良好的免疫逃逸性能而备受关注，而金纳米笼(AUNS)由于其光热效应和药物传递能力而被广泛应用于癌症治疗。红细胞膜涂层与AUNS的结合可能为肿瘤的靶向治疗提供一种有效的途径。然而，很少有报告显示这两种技术相结合的用途。在这里，我们设计了红细胞膜包裹的金纳米笼，用于靶向光热和化学治疗癌症。首先，抗EpCAM抗体被用来修饰红细胞膜以靶向4T1癌细胞。其次，将抗肿瘤药物紫杉醇(PTX)包埋在AUNS中。然后，将修饰后的红细胞膜包覆在AUNS上。这些新的纳米粒子被命名为EpCAM-RPAuns。我们表征了EpCAM-RPAuns通过暴露于近红外线照射而选择性靶向肿瘤的能力。实验结果表明，EpCAM-RPAuns可以有效地产生热疗，并将抗肿瘤药物PTX准确地输送到靶细胞。我们还验证了EPCAM-RAuns的体外生物相容性。通过将分子修饰的靶向红细胞膜与AUNS相结合，我们的方法为设计仿生纳米颗粒提供了一种新的途径，以增强纳米颗粒的表面功能。我们相信EpCAM-RPAuns可以潜在地应用于癌症的诊断和治疗。
Recently, red blood cell (RBC) membrane-coated nanoparticles have attracted much attention because of their excellent immune escapability; meanwhile, gold nanocages (AuNs) have been extensively used for cancer therapy due to their photothermal effect and drug delivery capability. The combination of the RBC membrane coating and AuNs may provide an effective approach for targeted cancer therapy. However, few reports have shown the utilization of combining these two technologies. Here, we design erythrocyte membrane-coated gold nanocages for targeted photothermal and chemical cancer therapy. First, anti-EpCam antibodies were used to modify the RBC membranes to target 4T1 cancer cells. Second, the antitumor drug paclitaxel (PTX) was encapsulated into AuNs. Then, the AuNs were coated with the modified RBC membranes. These new nanoparticles were termed EpCam-RPAuNs. We characterized the capability of the EpCam-RPAuNs for selective tumor targeting via exposure to near-infrared irradiation. The experimental results demonstrate that EpCam-RPAuNs can effectively generate hyperthermia and precisely deliver the antitumor drug PTX to targeted cells. We also validated the biocompatibility of the EpCam-RAuNs in vitro. By combining the molecularly modified targeting RBC membrane and AuNs, our approach provides a new way to design biomimetic nanoparticles to enhance the surface functionality of nanoparticles. We believe that EpCam-RPAuNs can be potentially applied for cancer diagnoses and therapies.