Label-Free Imaging of Membrane Potential Using Membrane Electromotility
Label-Free Imaging of Membrane Potential Using Membrane Electromotility
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DOI：
10.1016/j.bpj.2012.05.020
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发表时间：
2012-07-03
影响因子：
3.4
通讯作者：
Feld, Michael S.
中科院分区：
文献类型：
--
作者：
Oh, Seungeun;Fang-Yen, Christopher;Feld, Michael S.
在缺乏外源报告分子的情况下，电活动可能引起细胞结构的可观察到的变化。在这项工作中，我们报告了一种低相干干涉显微镜技术，该技术可以在没有外源标记的情况下检测与单个哺乳动物细胞膜电位变化相关的光信号。通过测量透射光的毫弧度相移，我们可以检测细胞膜电位的变化。我们发现观察到的光信号是由于膜电运动性引起的，这使得细胞在响应膜电位变化时发生变形。我们展示了在间隙连接耦合细胞网络中传播电刺激的宽视场成像。膜电致细胞变形可能是有用的电活动报告。
Electrical activity may cause observable changes in a cell's structure in the absence of exogenous reporter molecules. In this work, we report a low-coherence interferometric microscopy technique that can detect an optical signal correlated with the membrane potential changes in individual mammalian cells without exogenous labels. By measuring milliradian-scale phase shifts in the transmitted light, we can detect changes in the cells' membrane potential. We find that the observed optical signals are due to membrane electromotility, which causes the cells to deform in response to the membrane potential changes. We demonstrate wide-field imaging of the propagation of electrical stimuli in gap-junction-coupled cell networks. Membrane electromotility-induced cell deformation may be useful as a reporter of electrical activity.