Detection of lymph node metastasis of oesophageal cancer by RT-nested PCR for SCC antigen gene mRNA
Detection of lymph node metastasis of oesophageal cancer by RT-nested PCR for SCC antigen gene mRNA
复制标题
RT-巢式PCR检测SCC抗原基因mRNA检测食管癌淋巴结转移
DOI：
--
复制
发表时间：
2000
影响因子：
8.8
通讯作者：
Masayuki Imamura
中科院分区：
文献类型：
--
作者：
M. Kano;Y. Shimada;J. Kaganoi;Takaki Sakurai;Zhigang Li;Fumiaki Sato;G. Watanabe;Masayuki Imamura
随着分子生物学的发展，逆转录聚合酶链式反应(RT-PCR)已被应用于隐匿性淋巴结转移的检测，但对食道癌的研究报道较少。本研究旨在探讨以鳞状细胞癌(SCC)抗原基因为标记物，应用RT-巢式聚合酶链式反应检测食道癌隐匿性淋巴结转移的价值。SCC抗原已被广泛用作血清肿瘤标志物，并被报道为检测宫颈癌患者外周血中肿瘤细胞的靶基因。本文对14例食道癌患者的620个淋巴结进行了分析。RT-巢式聚合酶链式反应检测结果与病理和免疫组织化学检测结果进行比较。在敏感性试验中，RT-巢式聚合酶链式反应在107个外周血单核细胞中检测到101个鳞癌抗原产生细胞，而在43个对照淋巴结中未检测到。病理检查检出36个，免疫组织化学检查检出45个，RT-巢式聚合酶链式反应检出65个。RT-套式聚合酶链式反应检测到的淋巴结比病理或免疫组织化学检测的更多。鳞状细胞癌的敏感性和特异性似乎更高。SCC抗原基因是逆转录套式聚合酶链式反应检测食道癌隐匿性淋巴结转移较有用的标记物之一。©2000癌症研究活动
With recent development in molecular biology, reverse transcriptase polymerase chain reaction (RT-PCR) has been applied to detect occult lymph node metastasis, but there have been few reports concerning oesophageal cancer. The objective of this study is to investigate the usefulness of the squamous cell carcinoma (SCC) antigen gene as a marker with RT-nested PCR to detect occult lymph node metastases of oesophageal cancer. The SCC antigen has been widely used as a serum tumour marker and was reported as a target gene to detect tumour cells in peripheral blood in cervical cancer. In this study, 620 lymph nodes from 14 oesophageal cancer patients were analysed. The results of RT-nested PCR were compared with that of pathological and immunohistochemical examinations. In the test of sensitivity, the RT-nested PCR detected 101 of SCC antigen producing cells in 107 peripheral blood mononucleocytes and was not found in 43 control lymph nodes. The pathological examination, immunohistochemical examination and the RT-nested PCR detected 36, 45 and 65 nodes respectively. The RT-nested PCR detected statistically more lymph nodes than the pathological or immunohistochemical examination. The sensitivity and specificity seem higher in squamous cell carcinoma cases. The SCC antigen gene is one of the more useful markers for RT-nested PCR to detect occult lymph node metastases of oesophageal cancer. © 2000 Cancer Research Campaign
DOI：
10.1073/pnas.92.8.3147
发表时间：
1995-04-11
影响因子：
11.1
作者：
SCHNEIDER, SS;SCHICK, C;SILVERMAN, GA
通讯作者：
SILVERMAN, GA