SELECTIVE NEURONAL CELL ATTACHMENT TO A COVALENTLY PATTERNED MONOAMINE ON FLUORINATED ETHYLENE-PROPYLENE FILMS
SELECTIVE NEURONAL CELL ATTACHMENT TO A COVALENTLY PATTERNED MONOAMINE ON FLUORINATED ETHYLENE-PROPYLENE FILMS
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DOI：
10.1002/jbm.820270711
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发表时间：
1993-07-01
期刊：
影响因子：
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通讯作者：
AEBISCHER, P
中科院分区：
文献类型：
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作者：
RANIERI, JP;BELLAMKONDA, R;AEBISCHER, P
氟化乙烯丙烯膜（FEP）上的单胺的图案化共价表面加成在限定的培养基条件下控制细胞附着和分化。采用射频辉光放电（RFGD）工艺将FEP表面氟原子置换为羟基。然后通过聚合氨基丙基-三乙氧基硅烷（APTES）经由羟基官能团共价连接伯胺。确定细胞对APTES区域的选择性附着取决于白蛋白对图案化FEP膜的初始吸附。对于NB 2a神经母细胞瘤细胞系和原代大鼠内皮细胞，白蛋白被确定为增强细胞与APTES区域的附着并防止附着至未修饰的FEP区域。如果白蛋白没有被预吸附到膜上，则不会发生与修饰区的选择性附着。放射性标记的白蛋白与I-125表明，白蛋白的偏好吸附到单胺表面上的细胞优先附着。疏水力和离子力都对吸附过程有贡献。尽管可以通过白蛋白预吸附到表面来实现细胞选择性附着到图案化APTES区域，但除非向培养基中补充额外的血清成分，否则神经母细胞瘤细胞不会显着分化。
The patterned covalent surface addition of a monoamine to fluorinated ethylene propylene films (FEP) controls both cellular attachment and differentiation in defined media conditions. A radio frequency glow discharge (RFGD) process was used to replace FEP surface fluorine atoms with hydroxyl groups. The primary amine was then covalently attached by polymerizing aminopropyl-triethoxysilane (APTES) via the hydroxyl functionalities. The selective attachment of cells to the APTES regions was determined to be dependent upon the initial adsorption of albumin to the patterned FEP membrane. Albumin was determined to enhance cellular attachment to the APTES regions and prevent attachment to the unmodified FEP areas for both an NB2a neuroblastoma cell line and primary rat endothelial cells. If albumin were not preadsorbed onto the membrane, selective attachment to the modified regions would not occur. Radio-labeling albumin with I-125 demonstrated the preference of albumin for adsorption onto the monoamine surface where the cells preferentially attached. Both hydrophobic and ionic forces contributed to the adsorption process. Although selective cellular attachment to the patterned APTES regions could be achieved by albumin preadsorption to the surface, the neuroblastoma cells did not significantly differentiate unless additional serum components were supplemented to the media.