Stimulation of Formation of Inositol Phosphates in Primary Cultures of Bovine Adrenal Chromaffin Cells by Angiotensin II, Histamine, Bradykinin, and Carbachol
Stimulation of Formation of Inositol Phosphates in Primary Cultures of Bovine Adrenal Chromaffin Cells by Angiotensin II, Histamine, Bradykinin, and Carbachol
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血管紧张素 II、组胺、缓激肽和卡巴胆碱刺激牛肾上腺嗜铬细胞原代培养物中磷酸肌醇的形成
DOI：
10.1111/j.1471-4159.1988.tb01085.x
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发表时间：
1988
影响因子：
4.7
通讯作者：
M. Boarder
中科院分区：
文献类型：
--
作者：
R. Plevin;M. Boarder
摘要：组胺、缓激肽和血管紧张素 II 刺激肾上腺髓质释放儿茶酚胺。在这里，我们使用培养的牛肾上腺嗜铬细胞证明，这些激动剂以及卡巴胆碱（与六甲铵）刺激肌醇磷酸盐的产生。组胺反应对美吡拉敏敏感，涉及 H1 受体，而缓激肽的 EC50 低于 Met-Lys-缓激肽，而 [Des-Arg9]-缓激肽相对不活跃，涉及 BK-2 受体。测量了在锂存在下形成的总肌醇磷酸盐，其中组胺的反应最大。评估了嗜铬细胞和非嗜铬细胞在反应中的相对贡献。在每种情况下，嗜铬细胞都被发现对激动剂有反应。就组胺而言，反应仅发生在嗜铬细胞上。当肌醇磷酸盐累积超过 2 或 5 分钟且不存在锂时，在 Dowex 阴离子交换柱上分离时，缓激肽在肌醇三磷酸部分中产生最大的刺激，而组胺则产生更大的肌醇单磷酸累积。刺激 2 分钟后，对受刺激的肌醇三磷酸的异构体进行解析，在每种情况下，存在的主要异构体都是肌醇 1,3,4-三磷酸。讨论了对组胺和缓激肽的不同反应的两种假设。
Abstract: Histamine, bradykinin, and angiotensin II stimulate release of catecholamines from adrenal medulla. Here we show, using bovine adrenal chromaffin cells in culture, that these agonists as well as carbachol (with hexamethonium) stimulate production of inositol phosphates. The histamine response was mepyramine sensitive, implicating an H1 receptor, whereas bradykinin had a lower EC50 than Met‐Lys‐bradykinin, and [Des‐Arg9]‐bradykinin was relatively inactive, implicating a BK‐2 receptor. Total inositol phosphates formed in the presence of lithium were measured, with histamine giving the largest response. The relative contribution of chromaffin cells and nonchromaffin cells in the responses was assessed. In each case chromaffin cells were found to be responding to the agonists; in the case of histamine the response was solely on chromaffin cells. When the inositol phosphates accumulating over 2 or 5 min, with no lithium present, were separated on Dowex anion‐exchange columns, bradykinin gave the greatest stimulation in the inositol trisphosphate fraction, whereas histamine gave a larger inositol monophosphate accumulation. On resolution of the isomers of stimulated inositol trisphosphate after 2 min of stimulation, the principal isomer present was inositol 1,3,4‐trisphosphate in each case. Two hypotheses for the differential responses to histamine and bradykinin are discussed.
心脏的副交感神经效应器连接处。
影响因子：
21.1
作者：
Loffelholz,K;Pappano,AJ
通讯作者：
Pappano,AJ
佛波酯、甘油二酯和胆碱能激动剂对完整或洋地黄皂苷通透的肾上腺嗜铬细胞中蛋白激酶 C 的亚细胞分布的影响。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
TerBush,DR;Holz,RW
通讯作者：
Holz,RW